人β-防御素（hBD-1、hBD-2、hBD-3、hBD-4）在牙龈上皮中的表达。
The expression of human β-defensins (hBD-1, hBD-2, hBD-3, hBD-4) in gingival epithelia.
作者信息
Li Xinyi, Duan Dingyu, Yang Jingmei, Wang Panpan, Han Bo, Zhao Lei, Jepsen Soren, Dommisch Henrik, Winter Jochen, Xu Yi
机构信息
State Key Laboratory of Oral diseases, Chengdu, PR China; Department of Periodontology, College of Stomatology, Chongqing Medical University, Chongqing, PR China; Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, PR China.
State Key Laboratory of Oral diseases, Chengdu, PR China.
出版信息
Arch Oral Biol. 2016 Jun;66:15-21. doi: 10.1016/j.archoralbio.2016.01.012. Epub 2016 Jan 27.
OBJECTIVE
The aim of this study was to analyze and compare the expression patterns of human β-defensin-4 (hBD-4) with those of hBD-1, hBD-2, and hBD-3 in healthy and chronically inflamed gingival tissues.
MATERIALS AND METHODS
A total of 96 gingival samples were collected, comprising 46 biopsies from chronic periodontitis (CP) patients and 50 from individuals with healthy tissue. Of these, 21CP and 21 healthy samples were used to examine the protein expression of the hBDs by immunohistochemistry. The remaining 25CP and 29 healthy tissue samples were subjected to real-time reverse transcription polymerase chain reaction to quantify the mRNA expression of the hBDs.
RESULTS
All four types of β-defensin peptides were confined to the gingival epithelia. The percentage of samples positive for hBD-4 mRNA was significantly lower than the percentages for the other three β-defensins, both in healthy (p=0.003) and in inflamed (p=0.030) gingiva. However, in the case of the relative mRNA expression levels, that of hBD-4 was significantly higher than the levels for hBD-2 (healthy group: p<0.01; CP group: p<0.01) and hBD-3 (healthy group: p=0.004; CP group: p<0.01).
CONCLUSIONS
Our study demonstrates differential expression of hBD-1, hBD-2, hBD-3, and hBD-4 in healthy and CP gingiva.
目的
本研究旨在分析和比较人β-防御素-4（hBD-4）与hBD-1、hBD-2和hBD-3在健康和慢性炎症牙龈组织中的表达模式。
材料与方法
共收集96份牙龈样本，其中46份来自慢性牙周炎（CP）患者的活检组织，50份来自健康组织个体。其中，21份CP样本和21份健康样本用于通过免疫组织化学检测hBDs的蛋白表达。其余25份CP样本和29份健康组织样本进行实时逆转录聚合酶链反应，以定量hBDs的mRNA表达。
结果
所有四种类型的β-防御素肽均局限于牙龈上皮。hBD-4 mRNA阳性样本的百分比在健康牙龈（p=0.003）和炎症牙龈（p=0.030）中均显著低于其他三种β-防御素。然而，就相对mRNA表达水平而言，hBD-4的表达水平显著高于hBD-2（健康组：p<0.01；CP组：p<0.01）和hBD-3（健康组：p=0.004；CP组：p<0.01）。
结论
我们的研究表明hBD-1、hBD-2、hBD-3和hBD-4在健康和CP牙龈中存在差异表达。
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