Yang Xingbin, Zhao Yan, Lv You
College of Life Sciences, Key Laboratory of Ministry of Education for Medicinal Plant Resource and Natural Pharmaceutical Chemistry, Shaanxi Normal University, Xi'an 710062, China.
J Agric Food Chem. 2007 Jun 13;55(12):4684-90. doi: 10.1021/jf070241r. Epub 2007 May 19.
A simple and sensitive high-performance capillary electrophoresis (HPCE) method was designed for quantitative analysis of the component monosaccharides of an acidic polysaccharide extracted from pumpkin. In this method, the polysaccharide was hydrolyzed into component monosaccharides with 2.0 M trifluoroacetic acid at 100 degrees C for 6 h and then labeled with 1-phenyl-3-methyl-5-pyrazolone, and subsequently the labeled monosaccharide derivatives were separated by HPCE. As a result, glucose (21.7%) and glucuronic acid (18.9%) were identified to be the main component monosaccharides, followed by galactose (11.5%), arabinose (9.8%), xylose (4.4%), and rhamnose (2.8%). Furthermore, the pumpkin polysaccharide was also demonstrated to effectively inhibit the H2O2-caused decrease of cell viability, lactate dehydrogenase leakage, and malondialdehyde formation, and also reduced the H2O2-caused decline of superoxide dismutase activity and glutathione depletion in cultured mouse peritoneal macrophages, indicating that pumpkin polysaccharide possessed significant cytoprotective effect and antioxidative activity.
设计了一种简单灵敏的高效毛细管电泳（HPCE）方法，用于定量分析从南瓜中提取的酸性多糖的单糖成分。该方法中，多糖在100℃下用2.0 M三氟乙酸水解6小时生成单糖成分，然后用1-苯基-3-甲基-5-吡唑啉酮进行标记，随后通过HPCE分离标记的单糖衍生物。结果表明，葡萄糖（21.7%）和葡萄糖醛酸（18.9%）是主要的单糖成分，其次是半乳糖（11.5%）、阿拉伯糖（9.8%）、木糖（4.4%）和鼠李糖（2.8%）。此外，还证明南瓜多糖能有效抑制H2O2引起的细胞活力下降、乳酸脱氢酶泄漏和丙二醛形成，还能减轻H2O2引起的培养小鼠腹腔巨噬细胞中超氧化物歧化酶活性下降和谷胱甘肽消耗，表明南瓜多糖具有显著的细胞保护作用和抗氧化活性。
