Umavathi Saraswathi, Keerthika Madhayan, Gopinath Kasi, Kavitha Chandramohan, Romij Uddin Md, Alagumanian Shanmugam, Balalakshmi Chinnasamy
Department of Botany, Adhiyaman Arts and Science College for Women, Uthangarai 635207, Tamil Nadu, India.
School of Materials and Energy, Southwest University, Chongqing 400715, PR China.
Saudi J Biol Sci. 2021 Dec;28(12):6692-6700. doi: 10.1016/j.sjbs.2021.07.034. Epub 2021 Jul 20.
The pumpkin pulp contains a greater composition of edible polysaccharides and has reported with excellent biological applications. This research pertains to optimize the extraction of polysaccharides from the fleshy portion of the pumpkin using aqueous assisted extraction (AAE). The result showed that the optimal extraction condition of pumpkin polysaccharide was as follows: extraction temperature at 55 °C, pH 4.5, and enzyme concentration of 4000 µ/g for 80 min. Under the optimal extraction condition, the yield of pumpkin polysaccharide via AAE (15.4) was significantly higher. The biological activities of extracted polysaccharide including α-amylase inhibition (57.41% at 1000 µg/mL) and anti-inflammatory (50.41% at 25 µg/mL) activity increased significantly. Additionally, the antioxidant activities of extracted pumpkin polysaccharides including IC values of DPPH and ABTS were 59.87% and 58.74%, respectively. The pumpkin polysaccharide has maximum inhibitory effects against bacterial strains especially for than that of fungal strains. It is suggested that the aqueous assisted extraction of is a cost-effective promising method to decrease the processing time as well as enhancing extracted polysaccharide yield - times.
南瓜果肉含有较高比例的可食用多糖，并且据报道具有出色的生物学应用。本研究旨在优化使用水辅助提取（AAE）从南瓜肉质部分提取多糖的方法。结果表明，南瓜多糖的最佳提取条件如下：提取温度为55℃，pH值为4.5，酶浓度为4000µ/g，提取80分钟。在最佳提取条件下，通过AAE提取的南瓜多糖产量（15.4）显著更高。提取的多糖的生物活性，包括α-淀粉酶抑制活性（在1000µg/mL时为57.41%）和抗炎活性（在25µg/mL时为50.41%）显著增加。此外，提取的南瓜多糖的抗氧化活性，包括DPPH和ABTS的IC值分别为59.87%和58.74%。南瓜多糖对细菌菌株具有最大抑制作用，尤其是对[此处原文似乎不完整]比真菌菌株的抑制作用更强。建议水辅助提取是一种具有成本效益的有前景的方法，可减少加工时间并提高提取的多糖产量[此处原文“- times”表述似乎有误]。
