Iino Mayumi, Nomura Takahito, Tamaki Yuji, Yamada Yumiko, Yoneyama Koichi, Takeuchi Yasutomo, Mori Masaki, Asami Tadao, Nakano Takeshi, Yokota Takao
Center for Research on Wild Plants, Utsunomiya University, Utsunomiya 321-8505, Japan.
Phytochemistry. 2007 Jun;68(12):1664-73. doi: 10.1016/j.phytochem.2007.04.002. Epub 2007 May 23.
Progesterone is a mammalian gonadal hormone. In the current study, we identified and quantified progesterone in a range of higher plants by using GC-MS and examined its effects on the vegetative growth of plants. The growth of Arabidopsis (Arabidopsis thaliana) seedlings was promoted by progesterone at low concentrations but suppressed at higher concentrations under both light and dark growth conditions. The growth of the gibberellin-deficient mutant lh of pea (Pisum sativum) was also promoted by progesterone. An earlier study demonstrated that progesterone binds to MEMBRANE STEROID BINDING PROTEIN 1 (MSBP1) of Arabidopsis. In this work, we cloned the homologous genes of Arabidopsis, MSBP2 and STEROID BINDING PROTEIN (SBP), as well as of rice (Oryza sativa), OsMSBP1, OsMSBP2 and OsSBP and examined their expression in plant tissues. All of these genes, except OsMSBP1, were expressed abundantly in plant tissues. The roles of progesterone in plant growth were also discussed.
孕酮是一种哺乳动物性腺激素。在本研究中，我们通过气相色谱 - 质谱联用技术（GC-MS）鉴定并定量了多种高等植物中的孕酮，并研究了其对植物营养生长的影响。在光照和黑暗生长条件下，低浓度的孕酮促进拟南芥（Arabidopsis thaliana）幼苗的生长，但高浓度时则抑制其生长。豌豆（Pisum sativum）赤霉素缺陷型突变体lh的生长也受到孕酮的促进。早期研究表明，孕酮与拟南芥的膜类固醇结合蛋白1（MSBP1）结合。在本研究中，我们克隆了拟南芥的同源基因MSBP2和类固醇结合蛋白（SBP），以及水稻（Oryza sativa）的同源基因OsMSBP1、OsMSBP2和OsSBP，并检测了它们在植物组织中的表达。除OsMSBP1外，所有这些基因在植物组织中均大量表达。本文还讨论了孕酮在植物生长中的作用。
