Chao Jui-I, Perevedentseva Elena, Chung Pei-Hua, Liu Kuang-Kai, Cheng Chih-Yuan, Chang Chia-Ching, Cheng Chia-Liang
Institute of Pharmacology and Toxicology, Tzu-Chi University, Hualien, 970 Taiwan.
Biophys J. 2007 Sep 15;93(6):2199-208. doi: 10.1529/biophysj.107.108134. Epub 2007 May 18.
A novel method is proposed using nanometer-sized diamond particles as detection probes for biolabeling. The advantages of nanodiamond's unique properties were demonstrated in its biocompatibility, nontoxicity, easily detected Raman signal, and intrinsic fluorescence from its natural defects without complicated pretreatments. Carboxylated nanodiamond's (cND's) penetration ability, noncytotoxicity, and visualization of cND-cell interactions are demonstrated on A549 human lung epithelial cells. Protein-targeted cell interaction visualization was demonstrated with cND-lysozyme complex interaction with bacteria Escherichia coli. It is shown that the developed biomolecule-cND complex preserves the original functions of the test protein. The easily detected natural fluorescent and Raman intrinsic signals, penetration ability, and low cytotoxicity of cNDs render them promising agents in multiple medical applications.
提出了一种使用纳米级金刚石颗粒作为生物标记检测探针的新方法。纳米金刚石独特性质的优势体现在其生物相容性、无毒性、易于检测的拉曼信号以及天然缺陷产生的固有荧光，且无需复杂的预处理。在A549人肺上皮细胞上展示了羧化纳米金刚石（cND）的穿透能力、无细胞毒性以及cND与细胞相互作用的可视化。通过cND与溶菌酶复合物和大肠杆菌之间的相互作用，展示了蛋白质靶向的细胞相互作用可视化。结果表明，所开发的生物分子 - cND复合物保留了测试蛋白的原始功能。cND易于检测的天然荧光和拉曼固有信号、穿透能力以及低细胞毒性使其成为多种医学应用中有前景的试剂。
