体内RNA干扰突变体诱导表明了锥虫鞭毛功能在哺乳动物感染过程中的本质特性。
RNA interference mutant induction in vivo demonstrates the essential nature of trypanosome flagellar function during mammalian infection.
作者信息
Griffiths Samantha, Portman Neil, Taylor Philip R, Gordon Siamon, Ginger Michael L, Gull Keith
机构信息
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom.
出版信息
Eukaryot Cell. 2007 Jul;6(7):1248-50. doi: 10.1128/EC.00110-07. Epub 2007 May 18.
Abstract
We demonstrate that trypanosomes compromised in flagellar function are rapidly cleared from infected mice. Analysis of the PFR2 bloodstream RNA interference mutant revealed that defective cell motility occurred prior to cytokinesis failure. This validation provides a paradigm for the flagellum as a target for future assays and interventions against this human pathogen.
摘要
我们证明，鞭毛功能受损的锥虫会迅速从受感染小鼠体内清除。对PFR2血流RNA干扰突变体的分析表明，细胞运动缺陷发生在胞质分裂失败之前。这一验证为将鞭毛作为针对这种人类病原体的未来检测和干预靶点提供了范例。
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