Meng Qinglong, Pan Jingzhi, Liu Yajing, Chen Li, Ren Yueying
College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, Jilin 130118, P.R. China.
Tuberculous Meningitis Research Center, Infectious Disease Hospital, Changchun, Jilin 130123, P.R. China.
Exp Ther Med. 2018 Feb;15(2):1694-1701. doi: 10.3892/etm.2017.5568. Epub 2017 Nov 27.
, also known as Siberian ginseng, is widely distributed throughout northern Asia and used in traditional Chinese medicine; it has been reported to prevent a number of diseases. However, the association between the antitumour and immunostimulatory activities of polysaccharide extracted from (ASPS) remains to be elucidated. The aim of the present study was to investigate the anti-tumour and immunomodulatory effects of polysaccharide extracted from ASPS on Crocker sarcoma S, hepatic carcinoma H and uterine cervical carcinoma U tumour cell lines implanted in mice. High performance liquid chromatography, gas chromatography and infrared spectroscopy were used to analyse the monosaccharide composition of ASPS. The monosaccharide composition of ASPS (Arabic candy: Xylose: Glucose: Mannose) was 7.1:22.3:7.6:1.0. On day 0, female Kunming mice, were injected subcutaneously with 1×10 tumour cells in 0.2 ml. The inoculated mice were subsequently divided into five groups (10 mice/group) as follows: Model group, treated with normal saline; positive control group, treated with 30 mg/kg cyclophosphamide (CTX); and three treatment groups, treated with 200, 100 or 50 mg/kg ASPS. Non-inoculated mice were divided into the normal group, which was treated with normal saline, and the negative control group, which was treated with 200 mg/kg ASPS (n=10/group). CTX and ASPS were administered intragastrically once daily for 10 days. All mice were sacrificed on day 11. ASPS was observed to have an inhibitory effect on the growth of S, H and U cells in solid and ascites tumour-bearing mice. Serum interleukin (IL)-2 and IL-12 levels were significantly increased in S solid tumour-bearing mice treated with 200 or 100 mg/kg ASPS compared with mice in the normal, control and model groups (P<0.05), whereas serum IL-2 and IL-12 levels were significantly decreased in the cyclophosphamide treatment group compared with the normal, control and model groups (P<0.05). No significant difference in serum levels of tumour necrosis factor-α level was observed between any groups. In S and U solid tumour-bearing mice, no significant differences in serum levels of interferon (INF)-γ level in were observed between groups; however, in H solid tumour-bearing mice, treatment with ASPS significantly increased serum INF-γ compared with the positive control group (P<0.05). The results may provide a basis for the potential application of ASPS in clinical treatment for cancer.
，也被称为西伯利亚人参，广泛分布于整个北亚地区并用于传统中药；据报道它能预防多种疾病。然而，从 （ASPS） 中提取的多糖的抗肿瘤和免疫刺激活性之间的关联仍有待阐明。本研究的目的是调查从ASPS中提取的多糖对植入小鼠体内的克罗克肉瘤S、肝癌H和子宫颈癌U肿瘤细胞系的抗肿瘤和免疫调节作用。采用高效液相色谱、气相色谱和红外光谱分析ASPS的单糖组成。ASPS的单糖组成（阿拉伯糖：木糖：葡萄糖：甘露糖）为7.1:22.3:7.6:1.0。在第0天，将雌性昆明小鼠皮下注射0.2 ml含1×10个肿瘤细胞。随后将接种的小鼠分为五组（每组10只小鼠），如下：模型组，用生理盐水处理；阳性对照组，用30 mg/kg环磷酰胺（CTX）处理；三个治疗组，分别用200、100或50 mg/kg ASPS处理。未接种的小鼠分为正常组，用生理盐水处理，以及阴性对照组，用200 mg/kg ASPS处理（每组n = 10）。CTX和ASPS每天灌胃给药一次，持续10天。所有小鼠在第11天处死。观察到ASPS对实体瘤和腹水瘤荷瘤小鼠的S、H和U细胞生长有抑制作用。与正常、对照和模型组小鼠相比，用200或100 mg/kg ASPS处理的S实体瘤荷瘤小鼠血清白细胞介素（IL）-2和IL-12水平显著升高（P < 0.05），而环磷酰胺治疗组与正常、对照和模型组相比血清IL-2和IL-12水平显著降低（P < 0.05）。各组之间肿瘤坏死因子-α水平的血清水平未观察到显著差异。在S和U实体瘤荷瘤小鼠中，各组之间干扰素（INF）-γ水平的血清水平未观察到显著差异；然而，在H实体瘤荷瘤小鼠中，与阳性对照组相比，ASPS处理显著提高了血清INF-γ水平（P < 0.05）。这些结果可能为ASPS在癌症临床治疗中的潜在应用提供依据。
