Mittermayr Rainer, Osipov Anatoly, Piskernik Christina, Haindl Susanne, Dungel Peter, Weber Carina, Vladimirov Yuri A, Redl Heinz, Kozlov Andrey V
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology in the AUVA Research Center, Vienna, Austria.
Mol Med. 2007 Jan-Feb;13(1-2):22-9. doi: 10.2119/2006-00035.Mittermayr.
It has recently been shown that nitrosyl complexes of hemoglobin (NO-Hb) are sensitive to low-level blue laser irradiation, suggesting that laser irradiation can facilitate the release of biologically active nitric oxide (NO), which can affect tissue perfusion. The aim of this study was to evaluate the therapeutic value of blue laser irradiation for local tissue perfusion after surgical intervention. Blood was withdrawn from a rat, exposed to NO and infused back to the same rat or used for in vitro experiments. In vitro, an increase of NO-Hb levels (electron paramagnetic resonance spectroscopy) up to 15 microM in rat blood did not result in the release of detectable amounts of NO (NO selective electrode). Blue laser irradiation of NO-Hb in blood caused decomposition of NO-Hb complexes and release of free NO. Systemic infusion of NO-Hb in rats affected neither systemic circulation (mean arterial pressure) nor local tissue perfusion (Doppler blood flow imaging system). In contrast, a clear enhancement of local tissue perfusion was observed in epigastric flap when elevated NO-Hb levels in blood were combined with local He-Cd laser irradiation focused on the left epigastric artery. The enhancement of regional tissue perfusion was not accompanied by any detectable changes in systemic circulation. This study demonstrates that blue laser irradiation improves local tissue perfusion in a controlled manner stimulating NO release from NO-Hb complexes.
最近研究表明，血红蛋白的亚硝酰基复合物（NO-Hb）对低强度蓝光激光照射敏感，这表明激光照射可促进生物活性一氧化氮（NO）的释放，而NO可影响组织灌注。本研究旨在评估蓝光激光照射对手术干预后局部组织灌注的治疗价值。从大鼠体内取血，使其接触NO后再回输到同一大鼠体内，或用于体外实验。在体外，大鼠血液中NO-Hb水平（电子顺磁共振光谱法）升高至15微摩尔时，并未导致可检测量的NO释放（NO选择性电极法）。血液中NO-Hb经蓝光激光照射会导致NO-Hb复合物分解并释放出游离NO。向大鼠体内全身输注NO-Hb对全身循环（平均动脉压）和局部组织灌注（多普勒血流成像系统）均无影响。相比之下，当血液中NO-Hb水平升高并结合聚焦于左上腹动脉的局部氦镉激光照射时，可观察到上腹部皮瓣的局部组织灌注明显增强。局部组织灌注增强并未伴随全身循环出现任何可检测到的变化。本研究表明，蓝光激光照射可通过刺激NO从NO-Hb复合物中释放，以可控方式改善局部组织灌注。
