使用一维全相关谱（TOCSY）和一维旋转坐标系核欧沃豪斯效应谱（ROESY）实验对馏分成分进行表征。从龙舌兰属植物布里顿龙舌兰（Agave brittoniana Trel. spp. Brachypus）中分离得到四种新的螺旋甾烷皂苷。
Characterization of the fraction components using 1D TOCSY and 1D ROESY experiments. Four new spirostane saponins from Agave brittoniana Trel. spp. Brachypus.
作者信息
Macías Francisco A, Guerra José O, Simonet Ana M, Nogueiras Clara M
机构信息
Grupo de Alelopatía, Departamento de Química Orgánica, Facultad de Ciencias, Universidad de Cádiz, C/República Saharaui, s/n, 11510 Puerto Real, Cádiz, Spain.
出版信息
Magn Reson Chem. 2007 Jul;45(7):615-20. doi: 10.1002/mrc.2012.
A careful NMR analysis, especially 1D TOCSY and 1D ROESY, of two refined saponin fractions allowed us to determine the structures of four new saponins from a polar extract of the Agave brittoniana Trel. spp. Brachypus leaves. A full assignment of the 1H and 13C spectral data for these new saponins, agabrittonosides A-D (1-4), and one previously known saponin, karatavioside A (5) is reported. Their structures were established using a combination of 1D and 2D (1H, 1H-COSY, TOCSY, ROESY, g-HSQC, g-HMBC and g-HSQC-TOCSY) NMR techniques and ESI-MS. Moreover, the work represents a new approach to structural elucidation of saponins in refined fractions by NMR investigations.
对两个精制皂苷馏分进行仔细的核磁共振分析，尤其是一维全相关谱（1D TOCSY）和一维旋转框架 Overhauser 效应谱（1D ROESY），使我们能够从龙舌兰（Agave brittoniana Trel. spp. Brachypus）叶片的极性提取物中确定四种新皂苷的结构。本文报道了这些新皂苷——龙舌兰皂苷 A - D（1 - 4）以及一种先前已知的皂苷卡拉塔维皂苷 A（5）的氢谱（1H）和碳谱（13C）光谱数据的完整归属。它们的结构通过一维和二维（1H, 1H - 化学位移全相关谱（COSY）、全相关谱（TOCSY）、旋转框架 Overhauser 效应谱（ROESY）、梯度异核单量子相干谱（g - HSQC）、梯度异核多键相关谱（g - HMBC）和梯度异核单量子相干 - 全相关谱（g - HSQC - TOCSY））核磁共振技术以及电喷雾电离质谱（ESI - MS）相结合得以确定。此外，这项工作代表了一种通过核磁共振研究对精制馏分中的皂苷进行结构解析的新方法。
Zotero 插件