一种来自施氏龙舌兰的新型甾体皂苷。

A new steroidal saponin from Agave shrevei.

作者信息

Pereira Da Silva Bernadete, Valente Ana Paula, Paz Parente José

机构信息

Laboratório de Química de Plantas Medicinais, Núcleo de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro, PO Box 68045, CEP 21944-971, Rio de Janeiro, Brazil.

出版信息

Nat Prod Res. 2006 Apr;20(4):385-90. doi: 10.1080/14786410500045564.

DOI:10.1080/14786410500045564

PMID:16644534

Abstract

A new steroidal saponin was isolated from the leaves of Agave shrevei. Its structure was established as 3-[O-beta-D-glucopyranosyl-(1-->2)-O-[O-beta-D-glucopyranosyl-(1-->4)-O-[O-beta-D-glucopyranosyl-(1-->6)]-O-beta-D-glucopyranosyl-(1-->4)-beta-D-galactopyranosyl)-oxy]-(3beta,5alpha,25R)-spirostane. The structural identification was performed using detailed analyses of 1H- and 13C-NMR spectra including 2D NMR spectroscopic techniques (COSY, HETCOR, HMBC, and HMQC) and chemical conversions. The haemolytic activity of the steroidal saponin was evaluated using an in vitro assay.

摘要

从施氏龙舌兰的叶子中分离出一种新的甾体皂苷。其结构确定为3-[O-β-D-吡喃葡萄糖基-(1→2)-O-[O-β-D-吡喃葡萄糖基-(1→4)-O-[O-β-D-吡喃葡萄糖基-(1→6)]-O-β-D-吡喃葡萄糖基-(1→4)-β-D-吡喃半乳糖基)-氧基]-(3β,5α,25R)-螺旋甾烷。通过对1H-和13C-NMR光谱进行详细分析，包括二维NMR光谱技术（COSY、HETCOR、HMBC和HMQC）以及化学转化来进行结构鉴定。使用体外试验评估了该甾体皂苷的溶血活性。