对来自剑麻叶片的两种新呋甾烷醇皂苷的核磁共振研究。

The NMR studies on two new furostanol saponins from Agave sisalana leaves.

作者信息

Zou Peng, Fu Jing, Yu He-shui, Zhang Jie, Kang Li-ping, Ma Bai-ping, Yan Xian-zhong

机构信息

Beijing Institute of Radiation Medicine, Beijing 100850, People's Republic of China.

出版信息

Magn Reson Chem. 2006 Dec;44(12):1090-5. doi: 10.1002/mrc.1904.

DOI:10.1002/mrc.1904

PMID:16991112

Abstract

The detailed NMR studies and full assignments of the 1H and 13C spectral data for two new furostanol saponins isolated from Agave sisalana leaves are described. Their structures were established using a combination of 1D and 2D NMR techniques including 1H, 13C, 1H-1H COSY, TOCSY, HSQC, HMBC and HSQC-TOCSY, and also FAB-MS spectrometry and chemical methods. The structures were established as (25S)-26-(beta-D-glucopyranosyl)-22 xi-hydroxyfurost-12-one-3beta-yl-O-alpha-L-rhamnopyranosyl-(1-->4)-beta-D-glucopyranosyl-(1-->3)-O-[O-beta-D-glucopyranosyl-(1-->2)]-O-beta-D-glucopyranosyl-(1-->4)-beta-D-galacto- pyranoside (1) and (25S)-26-(beta-D-glucopyranosyl)-22xi-hydroxyfurost-5-en-12-one-3beta-yl-O-alpha-L-rhamno- pyranosyl-(1-->4)-beta-D-glucopyranosyl-(1-->3)-O-[O-beta-D-glucopyranosyl-(1-->2)]-O-beta-D-glucopyranosyl- (1-->4)-beta-D-galactopyranoside (2).

摘要

描述了从剑麻叶中分离得到的两种新呋甾烷醇皂苷的详细核磁共振研究以及1H和13C光谱数据的全归属。它们的结构通过结合一维和二维核磁共振技术确定，包括1H、13C、1H-1H COSY、TOCSY、HSQC、HMBC和HSQC-TOCSY，以及快原子轰击质谱法和化学方法。结构确定为(25S)-26-(β-D-吡喃葡萄糖基)-22ξ-羟基呋甾-12-酮-3β-基-O-α-L-吡喃鼠李糖基-(1→4)-β-D-吡喃葡萄糖基-(1→3)-O-[O-β-D-吡喃葡萄糖基-(1→2)]-O-β-D-吡喃葡萄糖基-(1→4)-β-D-吡喃半乳糖苷(1)和(25S)-26-(β-D-吡喃葡萄糖基)-22ξ-羟基呋甾-5-烯-12-酮-3β-基-O-α-L-吡喃鼠李糖基-(1→4)-β-D-吡喃葡萄糖基-(1→3)-O-[O-β-D-吡喃葡萄糖基-(1→2)]-O-β-D-吡喃葡萄糖基-(1→4)-β-D-吡喃半乳糖苷(2)。