Allen Timothy Craig, Fudala Rafal, Nash Sandra E, Kurdowska Anna
Department of Pathology, University of Texas Health Center, 11937 US Hwy 271, Tyler, TX 75708, USA.
Arch Pathol Lab Med. 2007 Mar;131(3):452-6. doi: 10.5858/2007-131-452-AAICVB.
Anti-interleukin 8 autoantibody:interleukin 8 (anti-IL-8 autoantibody:IL-8) complexes are present in lung fluids of patients with acute lung injury/acute respiratory distress syndrome (ALI/ARDS), and levels of these complexes correlate with progression to and the outcome of ARDS. Fc gammaRIIa, an immunoglobulin G (IgG) receptor, mediates proinflammatory activity of the complexes.
To evaluate lung tissues from patients with ARDS for presence of anti-IL-8 autoantibody:IL-8 complexes and to establish whether the complexes associate with Fc gammaRIIa.
Lung tissue sections from 3 patients with ARDS and sections of normal lung tissues from 3 patients were stained with antibodies against IL-8 and IgG to detect immune complexes and with antibody against Fc gammaRIIa. In some experiments, sections were blocked with anti-Fc gamma RIIa antibody before staining. Samples were analyzed using confocal microscopy.
Interleukin 8 costained with IgG and Fc gammaRIIa in lung tissues from patients with ARDS but not in control tissues, suggesting that anti-IL-8 autoantibody:IL-8 complexes are deposited in lungs of patients with ARDS via Fc gammaRIIa. Further, colocalization between IL-8 and Fc gammaRIIa could be blocked by anti-Fc gammaRIIa.
Our data demonstrate that anti-IL-8 autoantibody:IL-8 complexes are present in lung tissues of patients with ARDS, and are attached to Fc gammaRIIa.
抗白细胞介素8自身抗体：白细胞介素8（抗IL-8自身抗体：IL-8）复合物存在于急性肺损伤/急性呼吸窘迫综合征（ALI/ARDS）患者的肺液中，这些复合物的水平与ARDS的进展及预后相关。FcγRIIa是一种免疫球蛋白G（IgG）受体，介导这些复合物的促炎活性。
评估ARDS患者的肺组织中抗IL-8自身抗体：IL-8复合物的存在情况，并确定这些复合物是否与FcγRIIa相关。
对3例ARDS患者的肺组织切片和3例正常肺组织切片用抗IL-8和IgG抗体进行染色以检测免疫复合物，并用抗FcγRIIa抗体进行染色。在一些实验中，切片在染色前用抗FcγRIIa抗体封闭。使用共聚焦显微镜对样本进行分析。
在ARDS患者的肺组织中，白细胞介素8与IgG和FcγRIIa共染色，但在对照组织中未出现，这表明抗IL-8自身抗体：IL-8复合物通过FcγRIIa沉积在ARDS患者的肺中。此外，抗FcγRIIa可阻断IL-8与FcγRIIa之间的共定位。
我们的数据表明，抗IL-8自身抗体：IL-8复合物存在于ARDS患者的肺组织中，并与FcγRIIa结合。
