Verjovski-Almeida Sergio, Venancio Thiago M, Oliveira Katia C P, Almeida Giulliana T, DeMarco Ricardo
Laboratory of Gene Expression in Eukaryotes, Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes, 748, 05508-000 São Paulo, SP, Brazil.
Exp Parasitol. 2007 Nov;117(3):236-45. doi: 10.1016/j.exppara.2007.04.005. Epub 2007 Apr 21.
Recent advances in the study of Schistosoma mansoni genome and transcriptome have led to a better description of the S. mansoni gene complement. In this work, we report the design and use of a new S. mansoni 60-mer oligonucleotide microarray platform with approximately 44,000 probes, based on all publicly available cDNA sequence data for S. mansoni and Schistosoma japonicum. The large number of probes combined with the extensive sequence annotation available allowed a comprehensive approach, where most of the S. mansoni transcriptome is represented. Hybridization with adult worm RNA pointed to a set of genes transcriptionally active in this stage of the parasite's life cycle. Interestingly, a large proportion (43%) of genes for which transcription was detected in adults is comprised of "no match" genes, i.e. S. mansoni genes with unknown function and no identifiable orthologs in GenBank. Moreover, detection of bi-directional transcription for 7% of the active "no match" genes in adults leads us to hypothesize a widespread production of antisense RNA in S. mansoni, with possible regulatory roles.
曼氏血吸虫基因组和转录组研究的最新进展使得对曼氏血吸虫基因库有了更好的描述。在这项工作中，我们报告了一种基于曼氏血吸虫和日本血吸虫所有公开可用cDNA序列数据设计并使用的新型曼氏血吸虫60聚体寡核苷酸微阵列平台，该平台有大约44,000个探针。大量的探针与可用的广泛序列注释相结合，使得能够采用一种全面的方法，其中大部分曼氏血吸虫转录组都有体现。与成虫RNA的杂交表明在寄生虫生命周期的这个阶段有一组基因转录活跃。有趣的是，在成虫中检测到转录的基因中有很大一部分（43%）是“无匹配”基因，即功能未知且在GenBank中没有可识别直系同源物的曼氏血吸虫基因。此外，在成虫中7%的活跃“无匹配”基因检测到双向转录，这使我们推测曼氏血吸虫中广泛存在反义RNA的产生，并可能具有调控作用。
