Gobert Geoffrey N, McInnes Russell, Moertel Luke, Nelson Charles, Jones Malcolm K, Hu Wei, McManus Donald P
Queensland Institute of Medical Research and Australian Centre for International Health and Nutrition, Brisbane, Qld., Australia.
Exp Parasitol. 2006 Nov;114(3):160-72. doi: 10.1016/j.exppara.2006.03.003. Epub 2006 Apr 24.
We report the design, fabrication, and validation of a microarray covering the majority of the Schistosoma japonicum and Schistosoma mansoni transcriptomes. The oligonucleotide microarray contains 12,166 S. mansoni and 7055 S. japonicum target sequences. A confidence threshold of <or=0.001 (P value) was used in all analyses. The cross hybridization potential of the microarray was representative of 10,362 probes detected in both channels, while 12,052 probes hybridised to S. japonicum and 11,254 probes hybridised to S. mansoni. Differential hybridisation consisted of 3422 probes to S. mansoni mRNA and 3103 probes to S. japonicum mRNA. Important biological functions, such as transport, metabolism, immune evasion and host-parasite immunological interplay, cell communication, and sexual maturation are represented on this microarray. This is the first microarray commercially manufactured for studying schistosomes, and the large size and verified quality of the resource demonstrate its power for characterising the schistosome transcriptome.
我们报告了一种覆盖日本血吸虫和曼氏血吸虫大部分转录组的微阵列的设计、制作及验证情况。该寡核苷酸微阵列包含12,166个曼氏血吸虫和7055个日本血吸虫靶序列。所有分析均采用小于或等于0.001（P值）的置信阈值。微阵列的交叉杂交潜能由在两个通道中检测到的10,362个探针代表，其中12,052个探针与日本血吸虫杂交，11,254个探针与曼氏血吸虫杂交。差异杂交包括3422个针对曼氏血吸虫mRNA的探针和3103个针对日本血吸虫mRNA的探针。重要的生物学功能，如转运、代谢、免疫逃避和宿主 - 寄生虫免疫相互作用、细胞通讯以及性成熟等，都在该微阵列上有所体现。这是首个商业化生产用于研究血吸虫的微阵列，该资源的规模庞大且质量经过验证，证明了其在表征血吸虫转录组方面的能力。
