Evans Chris H, Palmer Glyn D, Pascher Arnulf, Porter Ryan, Kwong Francois N, Gouze Elvire, Gouze Jean-Noel, Liu Fangjun, Steinert Andre, Betz Oliver, Betz Volker, Vrahas Mark, Ghivizzani Steven C
Center for Molecular Orthopaedics, Harvard Medical School, Boston, Massachusetts 02115, USA.
Tissue Eng. 2007 Aug;13(8):1987-93. doi: 10.1089/ten.2006.0302.
Facilitated endogenous repair is a novel approach to tissue engineering that avoids the ex vivo culture of autologous cells and the need for manufactured scaffolds, while minimizing the number and invasiveness of associated clinical procedures. The strategy relies on harnessing the intrinsic regenerative potential of endogenous tissues using molecular stimuli, such as gene transfer, to initiate reparative processes in situ. In the simplest example, direct percutaneous injection of an osteogenic vector is used to stimulate bone healing. If necessary, additional progenitor cells and space-filling scaffolds can be provided by autologous bone marrow, muscle, fat, and perhaps other tissues. These can be harvested, processed, and reimplanted by simple, expedited, intraoperative procedures. Examples of repair of experimental osseous and osteochondral lesions in laboratory animals are described. If successful, these strategies will provide methods for tissue regeneration that are not only effective but also inexpensive, safe, and clinically expeditious. Although orthopaedic examples are given here, the technology should be more generally applicable.
促进内源性修复是一种组织工程的新方法，它避免了自体细胞的体外培养和制造支架的需求，同时将相关临床程序的数量和侵入性降至最低。该策略依靠利用分子刺激（如基因转移）来激发内源性组织的内在再生潜力，从而在原位启动修复过程。在最简单的例子中，直接经皮注射成骨载体用于刺激骨愈合。如有必要，自体骨髓、肌肉、脂肪以及其他可能的组织可提供额外的祖细胞和填充空间的支架。这些可以通过简单、快速的术中程序进行采集、处理和重新植入。文中描述了在实验动物中修复实验性骨和骨软骨损伤的例子。如果成功，这些策略将提供不仅有效而且廉价、安全和临床快捷的组织再生方法。尽管这里给出了骨科方面的例子，但该技术应具有更广泛的适用性。
