Li Yuezhi, Xu Tao, Guo Hui, Yang Huiling
College of Engineering and Technology, Shenzhen University, 518060 Shenzhen, China.
J Fluoresc. 2007 Jul;17(4):437-43. doi: 10.1007/s10895-007-0190-4. Epub 2007 May 23.
In this paper, the oxygen dependent phosphorescence quenching method is proposed to study the correlation between the phosphorescence lifetime and microvessel density in tumors. In the implementation, the S180 transplanted tumor in the mouse is used for the collection of the time-resolved phosphorescence, the tumor microvessel density is measured by immunohistochemical examination of FVIII, the correlation between microvessel density and phosphorescence lifetime is analyzed by multiple regression method. The results show the phosphorescence decay constant measured in tumors is enlarged in the tumor progression. Furthermore, the relative total microvessel area is positively correlative with the phosphorescence lifetime, which is estimated by a two dimension regression equation. It is concluded phosphorescence lifetime is a valuable indicator of angiogenesis during the tumor development.
本文提出了氧依赖磷光猝灭法来研究肿瘤中磷光寿命与微血管密度之间的相关性。在实验过程中，以小鼠体内的S180移植瘤作为时间分辨磷光的采集对象，通过对FVIII进行免疫组化检测来测定肿瘤微血管密度，采用多元回归方法分析微血管密度与磷光寿命之间的相关性。结果表明，在肿瘤进展过程中，肿瘤中测得的磷光衰减常数增大。此外，通过二维回归方程估计，相对总微血管面积与磷光寿命呈正相关。得出结论：磷光寿命是肿瘤发展过程中血管生成的一个有价值的指标。
