氧化DNA损伤、胃黏膜密度与幽门螺杆菌cagA、vacA和iceA基因型的相关性
Relationship among oxidative DNA damage, gastric mucosal density and the relevance of cagA, vacA and iceA genotypes of Helicobacter pylori.
作者信息
Ladeira Marcelo S P, Bueno Roberta C A, Dos Santos Bruna Fornazari, Pinto Carla L S, Prado Renato P, Silveira Marcela G, Rodrigues Maria A M, Bartchewsky Waldemar, Pedrazzoli José, Ribeiro Marcelo Lima, Salvadori Daisy M F
机构信息
Departamento de Clínica Médica, Laboratório de Biologia Molecular da Clínica Médica, UNESP, Botucatu, SP, Brazil.
出版信息
Dig Dis Sci. 2008 Jan;53(1):248-55. doi: 10.1007/s10620-007-9850-0. Epub 2007 May 23.
The aim of this study was to evaluate the relationship among oxidative DNA damage, density of Helicobacter pylori and the relevance of cagA, vacA and iceA genotypes of H. pylori. Gastric epithelial cells were isolated from 24 uninfected patients, 42 H. pylori infected patients with gastritis, and 61 patients with gastric cancer. Oxidative DNA damage was analyzed by the Comet assay, the density of H. pylori was measured by real-time polymerase chain reaction (PCR), and allelic variants of cagA, vacA and iceA were identified using the PCR. Infected patients by Helicobacter pylori cagA(+), vacAs1 m1 and iceA1 genotype showed higher levels of oxidative DNA damage than infected patients with H. pylori cagA(-), vacAs2 m2 and iceA2 genotypes and uninfected patients. Density of H. pylori did not influence oxidative DNA damage. Our results indicate that H. pylori genotype is more relevant than density for oxidative DNA damage.
本研究的目的是评估氧化性DNA损伤、幽门螺杆菌密度以及幽门螺杆菌cagA、vacA和iceA基因型之间的相关性。从24名未感染患者、42名幽门螺杆菌感染的胃炎患者和61名胃癌患者中分离胃上皮细胞。通过彗星试验分析氧化性DNA损伤，通过实时聚合酶链反应（PCR）测量幽门螺杆菌密度，并使用PCR鉴定cagA、vacA和iceA的等位基因变体。幽门螺杆菌cagA(+)、vacAs1 m1和iceA1基因型的感染患者比幽门螺杆菌cagA(-)、vacAs2 m2和iceA2基因型的感染患者以及未感染患者表现出更高水平的氧化性DNA损伤。幽门螺杆菌密度不影响氧化性DNA损伤。我们的结果表明，对于氧化性DNA损伤，幽门螺杆菌基因型比密度更具相关性。
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