Department of Clinical Microbiology and Immunology, Faculty of Pharmacy and Medical Laboratory Sciences, Third Military Medical University (Army Medical University), No. 30 Gaotanyan Street, Chongqing, 400038, People's Republic of China.
Department of Digestive Disease Center, The Third Affiliated Hospital of Chongqing Medical University (General Hospital), Chongqing, China.
BMC Biotechnol. 2020 Jun 22;20(1):33. doi: 10.1186/s12896-020-00624-z.
Helicobacter pylori (H. pylori) infection is a serious human health threat. The empiric H. pylori treatment paradigm guided by traditional testing technologies has led to antibiotic resistance. Here, we improved the qPCR method to provide technical support for precision H. pylori diagnosis and treatment.
Two pairs of primers and probes targeting the glmM gene were designed to detect H. pylori, and a multiplex qPCR method was established for virulence factor detection. Then, a rapid urease test (RUT), culturing and qPCR were performed on 141 specimens collected from Xinqiao Hospital of China in 2017 to evaluate the qPCR detection capability. Finally, the H. pylori infectious amount and virulence genes were detected by qPCR.
The H. pylori infectious amount and presence of H. pylori virulence factors showed complex correlations with gastric disease occurrence and development. The improved qPCR with good detection performance can be used for quantitative H. pylori detection and testing for the virulence genes vacA s1, vacA m1, cagA and babA2 simultaneously. These findings will provide valuable information for disease diagnosis and treatment.
幽门螺杆菌（H. pylori）感染是严重的人类健康威胁。传统检测技术指导的经验性 H. pylori 治疗范式导致了抗生素耐药性。在这里，我们改进了 qPCR 方法，为精准 H. pylori 诊断和治疗提供技术支持。
设计了针对 glmM 基因的两对引物和探针，建立了用于检测毒力因子的多重 qPCR 方法。然后，对 2017 年新桥医院采集的 141 份标本进行快速尿素酶试验（RUT）、培养和 qPCR，评估 qPCR 检测能力。最后，通过 qPCR 检测 H. pylori 感染量和毒力基因。
H. pylori 感染量和毒力因子的存在与胃疾病的发生和发展呈复杂的相关性。具有良好检测性能的改良 qPCR 可用于定量检测 H. pylori 并同时检测毒力基因 vacA s1、vacA m1、cagA 和 babA2。这些发现将为疾病诊断和治疗提供有价值的信息。
