Department of Microbiology, University of Texas Dental School, Houston, Texas 77025.
Infect Immun. 1970 Apr;1(4):380-6. doi: 10.1128/iai.1.4.380-386.1970.
The mouse-protective activity of Erysipelothrix rhusiopathiae culture supernatant fluids exists in a polydisperse form, ranging in density from aggregates which sediment at 10,000 x g for 3 hr to soluble units which will not sediment at 198,000 x g for 12 hr. A partially purified protective antigen has been isolated from the aggregates sedimented from a concentrate of the culture supernatant fluid at 20,000 x g for 3 hr. These aggregates contained the major protective antigen or antigens of E. rhusiopathiae, since, in addition to inducing active immunity, they adsorbed essentially all of the passively protecting antibody from rabbit antiserum produced by immunization with whole culture. The protective activity in these aggregates was destroyed by trypsin and greatly diminished by muramidase and heating at 64 C, but was not affected by lipase or ribonuclease.
猪丹毒杆菌培养上清液的抗鼠活性以多分散的形式存在，密度范围从在 10000xg 下沉淀 3 小时的聚集物到在 198000xg 下 12 小时不沉淀的可溶性单位。从 20000xg 下沉淀 3 小时的培养上清液浓缩物中分离出一种部分纯化的保护性抗原。这些聚集物含有猪丹毒杆菌的主要保护性抗原或抗原，因为它们不仅诱导主动免疫，而且还从用整个培养物免疫产生的兔抗血清中吸附了几乎所有的被动保护抗体。这些聚集物中的保护活性被胰蛋白酶破坏，被溶菌酶和 64°C 加热大大减弱，但不受脂肪酶或核糖核酸酶的影响。
