Shi Xiangyang, Wang Suhe, Meshinchi Sasha, Van Antwerp Mary E, Bi Xiangdong, Lee Inhan, Baker James R
Michigan Nanotechnology Institute for Medicine and Biological Sciences, University of Michigan, Ann Arbor, MI 48109, USA.
Small. 2007 Jul;3(7):1245-52. doi: 10.1002/smll.200700054.
We present a general approach for the targeting and imaging of cancer cells using dendrimer-entrapped gold nanoparticles (Au DENPs). Au DENPs were found to be able to covalently link with targeting and imaging ligands for subsequent cancer-cell targeting and imaging. The Au DENPs linked with defined numbers of folic acid (FA) and fluorescein isothiocyanate (FI) molecules are water soluble, stable, and biocompatible. In vitro studies show that the FA- and FI-modified Au DENPs can specifically bind to KB cells (a human epithelial carcinoma cell line) that overexpress high-affinity folate receptors and they are internalized dominantly into lysosomes of target cells within 2 h. These findings demonstrate that Au DENPs may serve as a general platform for cancer imaging and therapeutics.
我们展示了一种使用树枝状聚合物包裹的金纳米颗粒（Au DENPs）对癌细胞进行靶向和成像的通用方法。发现Au DENPs能够与靶向和成像配体共价连接，用于后续的癌细胞靶向和成像。与特定数量的叶酸（FA）和异硫氰酸荧光素（FI）分子连接的Au DENPs具有水溶性、稳定性和生物相容性。体外研究表明，FA和FI修饰的Au DENPs可以特异性结合过表达高亲和力叶酸受体的KB细胞（一种人上皮癌细胞系），并且在2小时内主要内化到靶细胞的溶酶体中。这些发现表明，Au DENPs可能作为癌症成像和治疗的通用平台。
