中间丝网络的柔软性、强度和自我修复能力。
Softness, strength and self-repair in intermediate filament networks.
作者信息
Wagner Oliver I, Rammensee Sebastian, Korde Neha, Wen Qi, Leterrier Jean-Francois, Janmey Paul A
机构信息
Department of Physiology, Institute for Medicine and Engineering, University of Pennsylvania, 1010 Vagelos Laboratories, 3340 Smith Walk, Philadelphia, PA 19104, USA.
出版信息
Exp Cell Res. 2007 Jun 10;313(10):2228-35. doi: 10.1016/j.yexcr.2007.04.025. Epub 2007 Apr 27.
One cellular function of intermediate filaments is to provide cells with compliance to small deformations while strengthening them when large stresses are applied. How IFs accomplish this mechanical role is revealed by recent studies of the elastic properties of single IF protein polymers and by viscoelastic characterization of the networks they form. IFs are unique among cytoskeletal filaments in withstanding large deformations. Single filaments can stretch to more than 3 times their initial length before breaking, and gels of IF withstand strains greater than 100% without damage. Even after mechanical disruption of gels formed by crossbridged neurofilaments, the elastic modulus of these gels rapidly recovers under conditions where gels formed by actin filaments are irreversibly ruptured. The polyelectrolyte properties of IFs may enable crossbridging by multivalent counterions, but identifying the mechanisms by which IFs link into bundles and networks in vivo remains a challenge.
中间丝的一个细胞功能是使细胞能够顺应小的变形，同时在受到大的应力时增强细胞。近期对单个中间丝蛋白聚合物弹性特性的研究以及对它们所形成网络的粘弹性表征揭示了中间丝是如何实现这一机械作用的。在承受大变形方面，中间丝在细胞骨架丝中是独一无二的。单根丝在断裂前可拉伸至其初始长度的三倍以上，中间丝凝胶能承受超过100%的应变而不受损伤。即使由交联神经丝形成的凝胶受到机械破坏，在肌动蛋白丝形成的凝胶不可逆破裂的条件下，这些凝胶的弹性模量仍能迅速恢复。中间丝的聚电解质特性可能使多价抗衡离子实现交联，但确定中间丝在体内连接成束和网络的机制仍然是一个挑战。
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