Jakab Ferenc, Horváth Gyozo, Ferenczi Emoke, Sebok Judit, Varecza Zoltán, Szucs György
Institute of Biology, University of Pécs, Pécs, Hungary.
Virus Res. 2007 Sep;128(1-2):149-52. doi: 10.1016/j.virusres.2007.04.015. Epub 2007 May 23.
Dobrava hantavirus (DOBV) belongs to the genus Hantavirus of the family Bunyaviridae, and is carried by yellow necked and striped field mice (Apodemus flavicollis and Apodemus agrarius), respectively. The aim of this study was to detect and genetically characterize new DOBV strains in rodents captured in the Transdanubian region of Hungary. Rodent corpses were dissected and lung tissues were used for hantavirus detection by SYBR Green-based real-time RT-PCR using specific primers located in the S-segment of the virus genome. A total of 22 captured animals of the Apodemus species were tested for the presence of DOBV. Three out of the 22 mice were positive. Phylogenetic and molecular sequence analyses showed that Hungarian DOBVs were most closely related to those viruses detected from A. agrarius mice in Slovenia. Based on our new data from the region we concluded that extended reservoir studies would be necessary in the future.
多布拉伐汉坦病毒（DOBV）属于布尼亚病毒科汉坦病毒属，分别由黄颈姬鼠和黑线姬鼠（黄颈姬鼠和黑线姬鼠）携带。本研究的目的是检测和基因鉴定在匈牙利多瑙河地区捕获的啮齿动物中的新型DOBV毒株。解剖啮齿动物尸体，使用位于病毒基因组S片段的特异性引物，通过基于SYBR Green的实时RT-PCR对肺组织进行汉坦病毒检测。总共对22只捕获的姬鼠属动物进行了DOBV检测。22只小鼠中有3只为阳性。系统发育和分子序列分析表明，匈牙利的DOBV与斯洛文尼亚从黑线姬鼠中检测到的病毒关系最为密切。根据我们从该地区获得的新数据，我们得出结论，未来有必要进行更广泛的宿主研究。
