Virological Research Group, Faculty of Sciences, Institute of Biology, University of Pécs, Ifjúság út 6, 7624 Pecs, Hungary.
Arch Virol. 2011 Sep;156(9):1655-60. doi: 10.1007/s00705-011-1013-0. Epub 2011 May 14.
Dobrava (DOBV) hantaviruses belong to the genus Hantavirus, family Bunyaviridae, and are carried by yellow-necked and striped field mice. The goal of this study was to detect DOBV using serological and genetic methods in Apodemus rodents in Hungary and in northern Croatia. During the study period, a total of 125 Apodemus sp. (67 A. agrarius, 58 A. flavicollis) were tested for the presence of hantaviruses, and 21 rodents (17%) were positive by rRT-PCR and/or ELISA. We conclude that the prevalence of DOBV is much higher than previously anticipated. The simultaneous use of molecular and serological techniques provides a highly reliable way to detect hantavirus infections.
多布拉瓦（DOBV）汉坦病毒属于汉坦病毒属、布尼亚病毒科，由黄颈田鼠和纹背田鼠携带。本研究的目的是使用血清学和遗传学方法在匈牙利和克罗地亚北部的小家鼠属啮齿动物中检测 DOBV。在研究期间，共检测了 125 只小家鼠属（67 只 A. agrarius，58 只 A. flavicollis）是否存在汉坦病毒，21 只啮齿动物（17%）通过 rRT-PCR 和/或 ELISA 呈阳性。我们得出结论，DOBV 的流行率远远高于先前的预期。分子和血清学技术的同时使用提供了一种高度可靠的方法来检测汉坦病毒感染。
