Mselle Teddy F, Meadows Sarah K, Eriksson Mikael, Smith Jennifer M, Shen Lilian, Wira Charles R, Sentman Charles L
Department of Microbiology and Immunology, Dartmouth Medical School, One Medical Center Drive, Lebanon, NH 03756, USA.
Clin Immunol. 2007 Jul;124(1):69-76. doi: 10.1016/j.clim.2007.04.008. Epub 2007 May 23.
In this study, we have analyzed the presence and subsets of NK cells throughout the tissues of the FRT. We demonstrate that there are NK cells in the various FRT tissues and that their phenotype and regulation are largely dependent upon the FRT tissue where they reside. NK cells in the Fallopian tube, endometrium, cervix, and ectocervix expressed CD9 while blood NK cells did not. We have also found that unique subsets of NK cells are in specific locations of the FRT. The NK cells in the lower reproductive tract did not express CD94, but they did express CD16. In contrast, NK cells in the upper FRT express high amounts of CD94 and CD69, but few NK cells expressed CD16. All of these FRT NK cells were able to produce IFN-gamma upon stimulation with cytokines. Furthermore, the number of NK cells varied with the menstrual cycle in the endometrium but not in the cervix or ectocervix. These data suggest that unique characteristics of the tissues may account of specific localization of different NK cell subsets.
在本研究中，我们分析了女性生殖道（FRT）各组织中自然杀伤（NK）细胞的存在情况及其亚群。我们证明，在FRT的各种组织中存在NK细胞，并且它们的表型和调控很大程度上取决于它们所在的FRT组织。输卵管、子宫内膜、子宫颈和子宫颈外口的NK细胞表达CD9，而血液中的NK细胞不表达。我们还发现，NK细胞的独特亚群存在于FRT的特定位置。下生殖道的NK细胞不表达CD94，但表达CD16。相比之下，上FRT的NK细胞表达大量的CD94和CD69，但很少有NK细胞表达CD16。所有这些FRT NK细胞在受到细胞因子刺激后都能够产生γ干扰素。此外，子宫内膜中NK细胞的数量随月经周期而变化，但子宫颈和子宫颈外口则不然。这些数据表明，组织的独特特性可能是不同NK细胞亚群特定定位的原因。
