Lebe Banu, Pabuççuoğlu Uğur, Ozer Erdener
Department of Pathology, Dokuz Eylül University School of Medicine, Inciralti, Izmir, Turkey.
Appl Immunohistochem Mol Morphol. 2007 Jun;15(2):160-4. doi: 10.1097/01.pai.0000209868.58699.64.
Familial acquired dysplastic nevi carry a risk for the development of melanoma. However, the results in various studies regarding the significance of sporadic dysplastic nevi as a precursor of malignant melanoma (MM), are controversial. The aim of this study is to investigate cyclin D1 expression and Ki67 proliferative index in a group of melanocytic lesions to address the biologic significance of sporadic dysplastic nevi in the progression of melanocytic lesions. Formalin-fixed paraffin-embedded material from 21 common melanocytic nevi, 42 dysplastic nevi, and 17 primary cutaneous MMs were examined. Standard streptavidin-biotin immunoperoxidase method was used for immunostaining with cyclin D1 and Ki-67 antibody. Nuclear cyclin D1 immunostaining was scored and Ki-67 proliferative index was calculated. Cyclin D1 expression was significantly higher in melanoma than those in other lesions. However, there was no significant difference between dysplastic nevi and common melanocytic nevi in terms of cyclin D1 expression. Ki-67 index was significantly higher in dysplastic nevi compared with common melanocytic nevi and to melanoma compared with dysplastic nevi. There was a significant positive correlation between cyclin D1 expression and Ki-67 proliferative index for each group. The present study indicates significant differences in cyclin D1 expressions and Ki-67 indices among melanocytic lesions. We think that dysplastic nevi are biologically separate from common melanocytic nevi in terms of proliferative activity. Additionally, our results suggest that cyclin D1 expression may be related to malignant phenotype and is associated with high proliferation rate in MM.
家族性获得性发育异常痣有发生黑色素瘤的风险。然而，关于散发性发育异常痣作为恶性黑色素瘤（MM）前驱病变的意义，各项研究结果存在争议。本研究的目的是调查一组黑素细胞病变中细胞周期蛋白D1的表达及Ki67增殖指数，以探讨散发性发育异常痣在黑素细胞病变进展中的生物学意义。对21例普通黑素细胞痣、42例发育异常痣和17例原发性皮肤MM的福尔马林固定石蜡包埋材料进行检测。采用标准链霉亲和素-生物素免疫过氧化物酶法，用细胞周期蛋白D1和Ki-67抗体进行免疫染色。对细胞核细胞周期蛋白D1免疫染色进行评分，并计算Ki-67增殖指数。细胞周期蛋白D1在黑色素瘤中的表达显著高于其他病变。然而，发育异常痣与普通黑素细胞痣在细胞周期蛋白D1表达方面无显著差异。与普通黑素细胞痣相比，发育异常痣的Ki-67指数显著更高；与发育异常痣相比，黑色素瘤的Ki-67指数显著更高。每组中细胞周期蛋白D1表达与Ki-67增殖指数之间均存在显著正相关。本研究表明黑素细胞病变在细胞周期蛋白D1表达和Ki-67指数方面存在显著差异。我们认为，就增殖活性而言，发育异常痣在生物学上与普通黑素细胞痣不同。此外，我们的结果表明，细胞周期蛋白D1表达可能与恶性表型相关，并且在MM中与高增殖率有关。
