Yamada Shigehito, Itoh Hitoshi, Uwabe Chigako, Fujihara Sachiko, Nishibori Chiaki, Wada Masaaki, Fujii Shingo, Shiota Kohei
Congenital Anomaly Research Center, Kyoto University Graduate School of Medicine, Kyoto, Japan.
Anat Rec (Hoboken). 2007 Mar;290(3):259-67. doi: 10.1002/ar.20427.
The developing heart and great vessels undergo drastic morphogenetic changes during the embryonic period. To analyze the normal and abnormal development of these organs, it is essential to visualize their structures in three and four dimensions, including the changes occurring with time. We have reconstructed the luminal structure of the hearts and great vessels of staged human embryos from serial histological sections to demonstrate their sequential morphological changes in three dimensions. The detailed structures of the embryonic heart and major arteries in normal and holoprosencephalic (HPE) human embryos could be reconstructed and visualized, and anatomical structures were analyzed using 3D images. By 3D analysis, cardiac anomalies such as double-outlet right ventricle and malrotation of the heart tube were identified in HPE embryos, which were not easily diagnosed by histological observation. Reconstruction and analysis of 3D images are useful for the study of anatomical structures of developing embryos and for identifying their abnormalities.
在胚胎期，发育中的心脏和大血管会经历剧烈的形态发生变化。为了分析这些器官的正常和异常发育情况，在三维和四维空间中可视化它们的结构至关重要，这包括随时间发生的变化。我们通过连续组织切片重建了不同发育阶段人类胚胎心脏和大血管的管腔结构，以展示其三维空间中的连续形态变化。正常和前脑无裂畸形（HPE）人类胚胎的胚胎心脏和主要动脉的详细结构得以重建并可视化，同时利用三维图像对解剖结构进行了分析。通过三维分析，在前脑无裂畸形胚胎中发现了诸如右心室双出口和心管旋转异常等心脏异常情况，而这些情况通过组织学观察不易诊断。三维图像的重建和分析对于研究发育中胚胎的解剖结构及其异常情况很有用。
