黄瓜花叶病毒RNA的所有四种RNA种类在5'端均含有7-甲基鸟苷。

Cucumber mosaic virus RNA contains 7-methyl guanosine at the 5'-terminus of all four RNA species.

作者信息

Symons R H

出版信息

Mol Biol Rep. 1975 Dec;2(4):277-85. doi: 10.1007/BF00357014.

Abstract

Purified cucumber mosaic virus (CMV) RNA was reacted with NaIO4 and then [3H]KBH4 to label ends containing exposed 2',3'-hydroxyls. The four major RNA species were then fractionated by polyacrylamide gel electrophoresis. Analysis of digests of these RNAs by ribonucleases T1 plus T2, alkaline phosphatease, snake venom diesterase and nucleotide pyrophosphatase by means of paper electrophoresis and ion-exchange chromatography indicated that the sequence m7G(5')ppp(5')N was present at the 5'-end of 75-92% of all RNA molecules. In addition, about 90% of the 3'-ends terminated in either adenosine or cytidine.

摘要

纯化的黄瓜花叶病毒（CMV）RNA与高碘酸钠反应，然后与[³H]硼氢化钾反应，标记含有暴露的2'，3'-羟基的末端。然后通过聚丙烯酰胺凝胶电泳对四种主要RNA种类进行分级分离。通过核糖核酸酶T1加T2、碱性磷酸酶、蛇毒二酯酶和核苷酸焦磷酸酶对这些RNA的消化产物进行纸电泳和离子交换色谱分析表明，所有RNA分子中75-92%的5'-末端存在序列m⁷G(5')ppp(5')N。此外，约90%的3'-末端以腺苷或胞苷终止。