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黄瓜花叶病毒RNA的所有四种RNA种类在5'端均含有7-甲基鸟苷。

Cucumber mosaic virus RNA contains 7-methyl guanosine at the 5'-terminus of all four RNA species.

作者信息

Symons R H

出版信息

Mol Biol Rep. 1975 Dec;2(4):277-85. doi: 10.1007/BF00357014.

DOI:10.1007/BF00357014
PMID:175260
Abstract

Purified cucumber mosaic virus (CMV) RNA was reacted with NaIO4 and then [3H]KBH4 to label ends containing exposed 2',3'-hydroxyls. The four major RNA species were then fractionated by polyacrylamide gel electrophoresis. Analysis of digests of these RNAs by ribonucleases T1 plus T2, alkaline phosphatease, snake venom diesterase and nucleotide pyrophosphatase by means of paper electrophoresis and ion-exchange chromatography indicated that the sequence m7G(5')ppp(5')N was present at the 5'-end of 75-92% of all RNA molecules. In addition, about 90% of the 3'-ends terminated in either adenosine or cytidine.

摘要

纯化的黄瓜花叶病毒（CMV）RNA与高碘酸钠反应，然后与[³H]硼氢化钾反应，标记含有暴露的2'，3'-羟基的末端。然后通过聚丙烯酰胺凝胶电泳对四种主要RNA种类进行分级分离。通过核糖核酸酶T1加T2、碱性磷酸酶、蛇毒二酯酶和核苷酸焦磷酸酶对这些RNA的消化产物进行纸电泳和离子交换色谱分析表明，所有RNA分子中75-92%的5'-末端存在序列m⁷G(5')ppp(5')N。此外，约90%的3'-末端以腺苷或胞苷终止。

相似文献

1
Cucumber mosaic virus RNA contains 7-methyl guanosine at the 5'-terminus of all four RNA species.黄瓜花叶病毒RNA的所有四种RNA种类在5'端均含有7-甲基鸟苷。
Mol Biol Rep. 1975 Dec;2(4):277-85. doi: 10.1007/BF00357014.
2
Cucumber mosaic virus-associated RNA 5. V. Extensive nucleotide sequence homology among CARNA 5 preparations of different CMV strains.黄瓜花叶病毒相关RNA 5。五、不同黄瓜花叶病毒株系的CARNA 5制剂间广泛的核苷酸序列同源性。
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The use of hybridization analysis with complementary DNA to determine the RNA sequence homology between strains of plant viruses: its application to several strains of cucumoviruses.利用互补DNA杂交分析确定植物病毒株系间的RNA序列同源性：其在几种黄瓜花叶病毒株系中的应用
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引用本文的文献

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Heterologous Replicase from Cucumoviruses can Replicate Viral RNAs, but is Defective in Transcribing Subgenomic RNA4A or Facilitating Viral Movement.黄瓜花叶病毒的异源复制酶能够复制病毒 RNA，但在转录亚基因组 RNA4A 或促进病毒运动方面存在缺陷。
Viruses. 2018 Oct 28;10(11):590. doi: 10.3390/v10110590.
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Anti-sense RNAs of cucumber mosaic virus in transgenic plants assessed for control of the virus.转黄瓜花叶病毒反义 RNA 的植物对病毒的控制作用评估。
Plant Mol Biol. 1988 Jul;11(4):463-71. doi: 10.1007/BF00039027.
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5'-terminal cap structure in eucaryotic messenger ribonucleic acids.

本文引用的文献

1
Blocked and unblocked 5' termini in reovirus genome RNA.呼肠孤病毒基因组RNA中封闭和未封闭的5'末端
J Virol. 1975 May;15(5):1057-64. doi: 10.1128/JVI.15.5.1057-1064.1975.
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Ribonuclease preparation for the base analysis of polyribonucleotides.用于多聚核糖核苷酸碱基分析的核糖核酸酶制剂。
Anal Biochem. 1966 Oct;17(1):135-42. doi: 10.1016/0003-2697(66)90016-9.
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[Photochemistry of the polynucleotides. II. Study of the fluorescence of nomonucleotides and dinucleotides at ordinary temperature].[多核苷酸的光化学。II. 常温下单核苷酸和二核苷酸荧光的研究]
真核生物信使核糖核酸中的5'-末端帽结构。
Microbiol Rev. 1980 Jun;44(2):175-205. doi: 10.1128/mr.44.2.175-205.1980.
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Nucleotide sequence of the satellite of peanut stunt virus reveals structural homologies with viroids and certain nuclear and mitochondrial introns.花生矮化病毒卫星核酸的核苷酸序列揭示了与类病毒以及某些核内和线粒体内含子的结构同源性。
Proc Natl Acad Sci U S A. 1985 May;82(10):3110-4. doi: 10.1073/pnas.82.10.3110.
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Minimal methylation of yeast messenger RNA.酵母信使核糖核酸的最小甲基化作用
Mol Biol Rep. 1976 Sep;3(1):9-17. doi: 10.1007/BF00357204.
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The terminal structures of feather keratin mRNA.羽毛角蛋白信使核糖核酸的末端结构
Mol Biol Rep. 1979 Aug 31;5(3):145-9. doi: 10.1007/BF00778413.
Biochim Biophys Acta. 1968 Dec 17;169(2):338-49.
4
The methylation of guanylyl-(3' replaced by 5')-uridine.鸟苷酰基-（3'被5'取代）-尿苷的甲基化
Biochim Biophys Acta. 1967 Nov 21;149(1):293-5.
5
The 3'-termini of bacteriophage Q-beta plus and minus strands.噬菌体Q-β正链和负链的3'末端。
J Mol Biol. 1970 Jul 28;51(2):215-24. doi: 10.1016/0022-2836(70)90138-5.
6
Cucumber mosaic virus contains a functionally divided genome.黄瓜花叶病毒含有一个功能上分开的基因组。
Virology. 1973 Jun;53(2):487-92. doi: 10.1016/0042-6822(73)90232-8.
7
Translation of brome mosaic viral ribonucleic acid in a cell-free system derived from wheat embryo.源自小麦胚的无细胞系统中雀麦花叶病毒核糖核酸的翻译
Proc Natl Acad Sci U S A. 1973 Jun;70(6):1799-803. doi: 10.1073/pnas.70.6.1799.
8
Modified nucleosides and bizarre 5'-termini in mouse myeloma mRNA.小鼠骨髓瘤mRNA中的修饰核苷和奇异的5'末端
Nature. 1975 May 1;255(5503):28-33. doi: 10.1038/255028a0.
9
5'-Terminal m-7G(5')ppp(5')G-m-p in vivo: identification in reovirus genome RNA.5'-末端 m-7G(5')ppp(5')G-m-p 在体内：呼肠孤病毒基因组 RNA 中的鉴定
Proc Natl Acad Sci U S A. 1975 Feb;72(2):742-5. doi: 10.1073/pnas.72.2.742.
10
Reovirus messenger RNA contains a methylated, blocked 5'-terminal structure: m-7G(5')ppp(5')G-MpCp-.呼肠孤病毒信使核糖核酸含有一个甲基化的、封闭的5'-末端结构：m-7G(5')ppp(5')G-MpCp- 。
Proc Natl Acad Sci U S A. 1975 Jan;72(1):362-6. doi: 10.1073/pnas.72.1.362.