5'-末端 m-7G(5')ppp(5')G-m-p 在体内：呼肠孤病毒基因组 RNA 中的鉴定
5'-Terminal m-7G(5')ppp(5')G-m-p in vivo: identification in reovirus genome RNA.
作者信息
Furuichi Y, Muthukrishnan S, Shatkin A J
出版信息
Proc Natl Acad Sci U S A. 1975 Feb;72(2):742-5. doi: 10.1073/pnas.72.2.742.
Abstract
Methylated reovirus mRNA was synthesized in vitro in the presence of S-adenosyl-L-[methyl-3H]-methionine. Viral genome double-stranded RNA that was uniformly labeled with 32-P was isolated from purified virions. The RNAs were mixed and their 5'-terminal structures compared by electrophoretic and chromatographic analyses after enzymatic digestion. Both the mRNA and the corresponding strand in the genome RNA contain m-7G(5')ppp(5')G-m-pCp, indicating that infected cells synthesize viral RNA with blocked, methylated 5' termini.
摘要
在S-腺苷-L-[甲基-³H]-甲硫氨酸存在的情况下，体外合成了甲基化呼肠孤病毒mRNA。从纯化的病毒粒子中分离出用³²P均匀标记的病毒基因组双链RNA。将这些RNA混合，并在酶消化后通过电泳和色谱分析比较它们的5'-末端结构。mRNA和基因组RNA中的相应链均含有m⁷G(5')ppp(5')G-m-pCp，表明受感染的细胞合成具有封闭的、甲基化5'末端的病毒RNA。
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