羽毛角蛋白信使核糖核酸的末端结构

The terminal structures of feather keratin mRNA.

作者信息

Morris C P, Rogers G E

出版信息

Mol Biol Rep. 1979 Aug 31;5(3):145-9. doi: 10.1007/BF00778413.

Abstract

Terminal labeling of embryonic feather keratin mRNA with [3H]KBH4 followed by digestion with ribonuclease T1 and T2, alkaline phosphatase, snake venom phosphodiesterase, and nucleotide pyrophosphatase and analysis of the products by high voltage paper electrophoresis, indicated the presence of the sequence m7G(5')ppp(5')N at the 5'-end of the mRNA. Ribonuclease T1 and A digests of the terminally labeled, and also of unlabeled mRNA followed by fractionation on denaturing polyacrylamide gels indicated the presence of polyadenylate tracts ranging in size from 45 to 165 nucleotide at the 3'-end of the mRNA.

摘要

用[3H]KBH4对胚胎羽毛角蛋白mRNA进行末端标记，随后用核糖核酸酶T1和T2、碱性磷酸酶、蛇毒磷酸二酯酶和核苷酸焦磷酸酶消化，并通过高压纸电泳分析产物，结果表明mRNA的5'-末端存在序列m7G(5')ppp(5')N。对末端标记的以及未标记的mRNA进行核糖核酸酶T1和A消化，然后在变性聚丙烯酰胺凝胶上进行分级分离，结果表明mRNA的3'-末端存在长度从45到165个核苷酸不等的聚腺苷酸序列。