母乳中HIV感染性病毒颗粒和BMCs的生存能力。
Viability of infectious viral particles of HIV and BMCs in breast milk.
作者信息
Yamaguchi Koushi, Sugiyama Takahiro, Takizawa Mari, Yamamoto Naoki, Honda Mitsuo, Natori Michiya
机构信息
Division of Maternal Medicine, Department of Perinatology, National Center for Child Health and Development, Okura 2-10-1, Setagaya-ku, Tokyo 157-8535, Japan.
出版信息
J Clin Virol. 2007 Jul;39(3):222-5. doi: 10.1016/j.jcv.2007.04.011. Epub 2007 May 29.
BACKGROUND
Infectious factors in breast milk such as viral particles and living infected cells are of prime importance in the transmission of HIV by breastfeeding.
OBJECTIVES
To perform effective approaches for reducing HIV transmission via breastfeeding, we investigated the biological importance of infectious viral particles and infected BMCs in breast milk.
STUDY DESIGN
Alteration of viral infectivity was monitored using a modified experimental infection assay that exploited the cytotoxicity of breast milk, and BMC viability was evaluated by flow-cytometric analysis.
RESULTS
Infectious viral particles were found to decrease time-dependently after contact with breast milk, whereas BMCs showed prolonged survival in breast milk.
CONCLUSIONS
The biological importance of infected BMCs in breast milk for the transmission of HIV via breastfeeding was considered.
背景
母乳中的感染性因素，如病毒颗粒和活的感染细胞，在母乳喂养传播HIV中至关重要。
目的
为了实施有效的减少母乳喂养传播HIV的方法，我们研究了母乳中感染性病毒颗粒和感染的BMCs的生物学重要性。
研究设计
使用改良的实验感染测定法监测病毒感染性的变化，该测定法利用了母乳的细胞毒性，并通过流式细胞术分析评估BMC的活力。
结果
发现感染性病毒颗粒在与母乳接触后随时间下降，而BMCs在母乳中存活时间延长。
结论
考虑了母乳中感染的BMCs在通过母乳喂养传播HIV中的生物学重要性。
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