Phan Huyen T T, Ellwood Simon R, Adhikari Kedar, Nelson Matthew N, Oliver Richard P
Australian Centre for Necrotrophic Fungal Pathogens, SABC, Department of Health Sciences, Murdoch University, Murdoch 6150, Australia.
DNA Res. 2007 Apr;14(2):59-70. doi: 10.1093/dnares/dsm009. Epub 2007 May 26.
We report the first genetic linkage map of white lupin (Lupinus albus L.). An F8 recombinant inbred line population developed from Kiev mutant x P27174 was mapped with 220 amplified fragment length polymorphism and 105 gene-based markers. The genetic map consists of 28 main linkage groups (LGs) that varied in length from 22.7 cM to 246.5 cM and spanned a total length of 2951 cM. There were seven additional pairs and 15 unlinked markers, and 12.8% of markers showed segregation distortion at P < 0.05. Syntenic relationships between Medicago truncatula and L. albus were complex. Forty-five orthologous markers that mapped between M. truncatula and L. albus identified 17 small syntenic blocks, and each M. truncatula chromosome aligned to between one and six syntenic blocks in L. albus. Genetic mapping of three important traits: anthracnose resistance, flowering time, and alkaloid content allowed loci governing these traits to be defined. Two quantitative trait loci (QTLs) with significant effects were identified for anthracnose resistance on LG4 and LG17, and two QTLs were detected for flowering time on the top of LG1 and LG3. Alkaloid content was mapped as a Mendelian trait to LG11.
我们报道了首个白羽扇豆（Lupinus albus L.）的遗传连锁图谱。利用基辅突变体与P27174杂交构建的F8重组自交系群体，采用220个扩增片段长度多态性标记和105个基于基因的标记进行图谱构建。遗传图谱由28个主要连锁群（LGs）组成，长度从22.7厘摩到246.5厘摩不等，总长度为2951厘摩。另有7对标记和15个不连锁标记，12.8%的标记在P < 0.05时表现出分离畸变。蒺藜苜蓿与白羽扇豆之间的共线性关系复杂。在蒺藜苜蓿和白羽扇豆之间定位的45个直系同源标记鉴定出17个小的共线性区段，蒺藜苜蓿的每条染色体与白羽扇豆中1至6个共线性区段对齐。对三个重要性状进行遗传作图：炭疽病抗性、开花时间和生物碱含量，从而确定了控制这些性状的基因座。在LG4和LG17上鉴定出两个对炭疽病抗性有显著影响的数量性状基因座（QTLs），在LG1和LG3顶部检测到两个开花时间的QTLs。生物碱含量作为孟德尔性状定位到LG11上。
