Department of Genetics, Plant Breeding and Seed Production, Wroclaw University of Environmental and Life Sciences, Plac Grunwaldzki 24A, 50-363, Wrocław, Poland.
Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479, Poznań, Poland.
J Appl Genet. 2020 Dec;61(4):531-545. doi: 10.1007/s13353-020-00585-1. Epub 2020 Sep 23.
White lupin (Lupinus albus L.) is a high-protein grain legume crop, grown since ancient Greece and Rome. Despite long domestication history, its cultivation remains limited, partly because of susceptibility to anthracnose. Only some late-flowering, bitter, low-yielding landraces from Ethiopian mountains displayed resistance to this devastating disease. The resistance is controlled by various genes, thereby complicating the breeding efforts. The objective of this study was developing tools for molecular tracking of Ethiopian resistance genes based on genotyping-by-sequencing (GBS) data, envisaging linkage mapping and genomic selection approaches. Twenty GBS markers from two major quantitative trait loci (QTLs), antr04_1/antr05_1 and antr04_2/antr05_2, were converted to PCR-based markers using assigned transcriptome sequences. Newly developed markers improved mapping resolution around both anthracnose resistance loci, providing more precise QTL estimation. PCR-based screening of diversified domesticated and primitive germplasm revealed the high specificity of two markers for the antr04_1/antr05_1 locus (TP222136 and TP47110) and one for the antr04_2/antr05_2 locus (TP338761), highlighted by simple matching coefficients of 0.96 and 0.89, respectively. Moreover, a genomic selection approach based on GBS data of a recombinant inbred line mapping population was assessed, providing an average predictive ability of 0.56. These tools can be used for preselection of candidate white lupin germplasm for anthracnose resistance assays.
白 Lupinus albus L. 是一种高蛋白谷物豆科作物，自古希腊和罗马时代就开始种植。尽管经过了长期的驯化历史，但它的种植仍然受到限制，部分原因是对炭疽病的敏感性。只有一些来自埃塞俄比亚山区的晚开花、苦味、低产的地方品种表现出对这种毁灭性疾病的抗性。这种抗性由各种基因控制，从而使育种工作变得复杂。本研究的目的是开发基于基因分型测序 (GBS) 数据的分子追踪埃塞俄比亚抗性基因的工具，设想连锁图谱和基因组选择方法。从两个主要的数量性状位点 (QTL) antr04_1/antr05_1 和 antr04_2/antr05_2 中选择了 20 个 GBS 标记，利用分配的转录组序列将其转化为基于 PCR 的标记。新开发的标记提高了两个炭疽病抗性基因座附近的作图分辨率，提供了更精确的 QTL 估计。对多样化的驯化和原始种质资源进行的基于 PCR 的筛选显示，两个标记对 antr04_1/antr05_1 基因座 (TP222136 和 TP47110) 和一个标记对 antr04_2/antr05_2 基因座 (TP338761) 的特异性较高，简单匹配系数分别为 0.96 和 0.89。此外，还评估了基于重组自交系作图群体 GBS 数据的基因组选择方法，提供了平均预测能力为 0.56。这些工具可用于炭疽病抗性测定中白 Lupinus albus L. 候选种质资源的预筛选。
