Surma Anna, Książkiewicz Michał, Bielski Wojciech, Kozak Bartosz, Galek Renata, Rychel-Bielska Sandra
Department of Gene Structure and Function, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479, Poznań, Poland.
Department of Genetics and Plant Breeding, Poznań University of Life Sciences, Dojazd 11, 60-631, Poznań, Poland.
Sci Rep. 2025 Jan 21;15(1):2659. doi: 10.1038/s41598-025-86482-1.
White lupin (Lupinus albus L.) is an ancient grain legume that is still undergoing improvement of domestication traits, including vernalization-responsiveness, providing frost tolerance and preventing winter flowering in autumn-sowing agriculture, and vernalization-independence, conferring drought escape by rapid flowering in spring-sowing. A recent genome-wide association study highlighted several loci significantly associated with the most contrasting phenotypes, including deletions in the promoter of the FLOWERING LOCUS T homolog, LalbFTc1, and some DArT-seq/silicoDArT loci. The present study aimed to develop and validate a versatile PCR marker array enabling molecular selection of spring- and winter-type white lupin ecotypes. Candidate DArT-seq and silicoDArT loci were transformed into cleaved amplified polymorphic sequence (CAPS) or derived CAPS markers. Developed markers, together with those previously published for LalbFTc1 INDELs and quantitative trait loci from linkage maps, were implemented for screening of white lupin germplasm panel subjected to 2-year phenotyping of phenology traits. Three DArT-seq, two silicoDArT and seven LalbFTc1 INDEL markers were positively validated, constituting a convenient PCR-based marker assay for rapid and accurate reselection of white lupin germplasm towards early flowering and thermoneutrality or late flowering and vernalization-responsiveness, as well as for tracking high genetic and phenotypic diversity within white lupin landraces, revealed in the present study.
白羽扇豆（Lupinus albus L.）是一种古老的谷物豆类，其驯化性状仍在不断改良，包括春化反应，即在秋播农业中提供抗冻性并防止冬季开花；以及春化不敏感性，即在春播中通过快速开花实现避旱。最近一项全基因组关联研究突出了几个与最显著对比表型显著相关的位点，包括开花位点T同源基因LalbFTc1启动子中的缺失，以及一些DArT-seq/电子DArT位点。本研究旨在开发并验证一种通用的PCR标记阵列，用于对春型和冬型白羽扇豆生态型进行分子选择。将候选DArT-seq和电子DArT位点转化为酶切扩增多态性序列（CAPS）或衍生CAPS标记。将开发的标记与先前发表的LalbFTc1插入缺失标记以及连锁图谱中的数量性状位点一起，用于对白羽扇豆种质资源库进行筛选，该种质资源库已进行了两年的物候性状表型分析。三个DArT-seq、两个电子DArT和七个LalbFTc1插入缺失标记得到了阳性验证，构成了一种便捷的基于PCR的标记检测方法，可用于快速准确地重新选择白羽扇豆种质资源，使其具有早花和热中性或晚花和春化反应特性，以及用于追踪本研究中揭示的白羽扇豆地方品种内的高遗传和表型多样性。
