Boldur I, Kahana H, Kazak R, Cwikel B, Sarov I
Department of Microbiology, Assaf Harofeh Medical Center, Tel-Aviv University, Zerifin, Israel.
J Clin Pathol. 1991 Nov;44(11):932-5. doi: 10.1136/jcp.44.11.932.
The immune response to individual major antigens of Legionella bozemanii was studied in 67 sera from 26 inpatients with febrile disease using immunoblotting techniques. All the patients had fever of unknown origin and showed strong serological reactions to L bozemanii that cross-reacted with Rickettsia typhi, as determined by a standard indirect microimmunofluorescence assay. Sera analysed by western blotting reacted with 12-14 molecular components of L bozemanii with apparent molecular weights ranging from 14,000 to 94,000 daltons. These reactions compared well with a reference system using antisera of rabbits immunised with the same strain of Legionella. The three major cross-reactive components of R typhi were polypeptide antigens of 94,000, 67,000 and 43,000 daltons. It is concluded that western blotting can help in the differential diagnosis of patients with fever of unknown origin whose sera cross-react to L bozemanii and R typhi.
运用免疫印迹技术，对26例发热性疾病住院患者的67份血清中针对博兹曼军团菌各主要抗原的免疫反应进行了研究。所有患者均有不明原因发热，且经标准间接微量免疫荧光测定法测定，其血清对博兹曼军团菌呈现强烈血清学反应，并与伤寒立克次体发生交叉反应。经蛋白质印迹法分析的血清与博兹曼军团菌的12 - 14种分子成分发生反应，这些成分的表观分子量在14,000至94,000道尔顿之间。这些反应与使用同一株军团菌免疫的兔抗血清的参考系统结果吻合良好。伤寒立克次体的三种主要交叉反应成分是分子量为94,000、67,000和43,000道尔顿的多肽抗原。结论是，蛋白质印迹法有助于对血清与博兹曼军团菌和伤寒立克次体发生交叉反应的不明原因发热患者进行鉴别诊断。
