Hindahl M S, Iglewski B H
Infect Immun. 1986 Jan;51(1):94-101. doi: 10.1128/iai.51.1.94-101.1986.
Outer membranes were isolated from eight serogroups of L. pneumophila and five other Legionella species. The protein composition of the membranes was characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A single, disulfide stabilized protein with a molecular size of 29,000 to 30,000 daltons was found to be the major outer membrane protein (MOMP) of all the serogroups. The equivalent of the L. pneumophila MOMP was not observed in any of the other Legionella species examined. Silver staining of sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels revealed distinctive patterns for each serogroup and other Legionella species that were not observed by staining with Coomassie blue and may result from the presence of lipopolysaccharide in the membrane preparations. The MOMP from serogroup 1 was isolated by exposing crude peptidoglycan to detergent in the presence of heat and reducing agent and was found to be tightly associated with lipopolysaccharide. Antibodies to this complex were used to probe the outer membranes of the remaining, L. pneumophila serogroups and other Legionella species by Western blotting. Serogroup 1 anti-MOMP antibodies were found to react with the MOMP from the remaining seven serogroups examined, whereas antibodies directed against the lipopolysaccharide of serogroup 1 only reacted with lipopolysaccharide from two of the remaining seven serogroups.
从嗜肺军团菌的八个血清群和其他五种军团菌中分离出外膜。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳对外膜的蛋白质组成进行表征。发现一种分子量为29,000至30,000道尔顿的单一二硫键稳定蛋白是所有血清群的主要外膜蛋白（MOMP）。在所检测的其他军团菌物种中均未观察到与嗜肺军团菌MOMP相当的蛋白。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳凝胶的银染色揭示了每个血清群和其他军团菌物种的独特模式，这些模式在用考马斯亮蓝染色时未观察到，可能是由于膜制剂中存在脂多糖所致。通过在加热和还原剂存在的情况下将粗肽聚糖暴露于去污剂中，分离出了血清群1的MOMP，发现它与脂多糖紧密结合。用针对该复合物的抗体通过蛋白质印迹法检测其余嗜肺军团菌血清群和其他军团菌物种的外膜。发现血清群1抗MOMP抗体与所检测的其余七个血清群的MOMP发生反应，而针对血清群1脂多糖的抗体仅与其余七个血清群中的两个血清群的脂多糖发生反应。
