Moore W E, Moore L H, Ranney R R, Smibert R M, Burmeister J A, Schenkein H A
Department of Anaerobic Microbiology, Virginia Polytechnic Institute and State University, Blacksburg.
J Clin Periodontol. 1991 Nov;18(10):729-39. doi: 10.1111/j.1600-051x.1991.tb00064.x.
20 adult periodontitis (AP) subjects were examined every 2 to 4 months and microbiological samples were collected and cultured when 2 mm or more loss of attachment (active sites) was detected by 2 examiners. Similar sites in which no progressive destruction was observed (control sites) also were sampled in the same subjects. By lambda-analysis, there was no statistically significant difference in floras of active (42 sites from 12 subjects) and control (36 sites from 12 subjects) sites or between the floras of the active and control sites and of 63 samples from 22 AP subjects that were examined previously in a cross-sectional study. By paired t test, no microbial species had a significantly greater association with active than with control sites. The only species that were detected in one or more samples from all subjects with active sites were Wolinella recta, Fusobacterium nucleatum, and Peptostreptococcus micros. Porphyromonas gingivalis and 9 other taxa were isolated from one-half or more of the persons with active sites. The composition of microbiological floras of all periodontitis samples was statistically significantly different from that of subjects with healthy gingiva. The composition of microfloras of sites in subjects with naturally-occurring gingivitis was intermediate between that of subjects with healthy gingiva and that of active and control sites in AP subjects.
每2至4个月对20名成人牙周炎（AP）患者进行检查，当两名检查者检测到附着丧失2毫米或更多（活动部位）时，采集微生物样本并进行培养。在同一受试者中，还对未观察到进行性破坏的类似部位（对照部位）进行采样。通过λ分析，活动部位（来自12名受试者的42个部位）和对照部位（来自12名受试者的36个部位）的菌群之间，以及活动部位和对照部位的菌群与之前在横断面研究中检查的22名AP受试者的63个样本的菌群之间，在统计学上没有显著差异。通过配对t检验，没有微生物物种与活动部位的关联比与对照部位的关联显著更强。在所有有活动部位的受试者的一个或多个样本中检测到的唯一物种是直形沃氏菌、具核梭杆菌和微小消化链球菌。牙龈卟啉单胞菌和其他9个分类群从一半或更多有活动部位的人中分离出来。所有牙周炎样本的微生物菌群组成与健康牙龈受试者的菌群组成在统计学上有显著差异。自然发生牙龈炎受试者部位的微生物菌群组成介于健康牙龈受试者和AP受试者活动部位及对照部位的微生物菌群组成之间。
