• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Reliability of gender determination using the polymerase chain reaction (PCR) for single cells.

作者信息

Strom C M, Rechitsky S, Verlinsky Y

机构信息

Reproductive Genetics Institute, Illinois Masonic Medical Center, Chicago 60657.

出版信息

J In Vitro Fert Embryo Transf. 1991 Aug;8(4):225-9. doi: 10.1007/BF01130810.

DOI:10.1007/BF01130810
PMID:1753170
Abstract

Contamination with extraneous DNA sequences is a frequent problem when performing PCR analysis of single cells. This report describes our experience with eliminating contaminating DNA sequences from PCR reagents for the purposes of gender identification. We have used amplification of Y-specific sequences to identify the gender of single human amniocytes. Female cells consistently showed no Y-specific bands but only 80% of male cells showed the expected intense Y-specific band. This phenomenon could lead to incorrect gender identification of single cells. We developed a technique of simultaneous amplification of X- and Y-specific sequences to prevent misdiagnosis because of failed PCR, which allows accurate preimplantation gender determination for women at risk for conceiving children with X-linked genetic diseases. We analyzed the gender of 141 consecutive single cells in a blinded manner without a single incorrect gender assignment.

摘要

相似文献

1
Reliability of gender determination using the polymerase chain reaction (PCR) for single cells.
J In Vitro Fert Embryo Transf. 1991 Aug;8(4):225-9. doi: 10.1007/BF01130810.
2
Identifying the sex of human preimplantation embryos in X-linked disease: amplification efficiency of a Y-specific alphoid repeat from single blastomeres with two lysis protocols.在X连锁疾病中鉴定人类植入前胚胎的性别：两种裂解方案对单个卵裂球中Y特异性α卫星重复序列的扩增效率
J Assist Reprod Genet. 1996 Feb;13(2):125-32. doi: 10.1007/BF02072533.
3
Preimplantation prevention of X-linked disease: reliable and rapid sex determination of single human cells by restriction analysis of simultaneously amplified ZFX and ZFY sequences.植入前X连锁疾病的预防：通过对同时扩增的ZFX和ZFY序列进行限制性分析，对单个人类细胞进行可靠且快速的性别鉴定。
Hum Mol Genet. 1993 Aug;2(8):1187-91. doi: 10.1093/hmg/2.8.1187.
4
Reliable gender screening for human preimplantation embryos, using multiple DNA target-sequences.利用多个DNA靶序列对人类植入前胚胎进行可靠的性别筛查。
Hum Reprod. 1992 Oct;7(9):1304-13. doi: 10.1093/oxfordjournals.humrep.a137846.
5
Amplification of X- and Y-chromosome-specific regions from single human blastomeres by polymerase chain reaction for sexing of preimplantation embryos.通过聚合酶链反应从单个人类卵裂球扩增X和Y染色体特异性区域用于植入前胚胎的性别鉴定。
Hum Reprod. 1994 Apr;9(4):716-20. doi: 10.1093/oxfordjournals.humrep.a138576.
6
Influence of mosaicism on sexing of human preembryos detected by the polymerase chain reaction.嵌合现象对通过聚合酶链反应检测的人类胚胎前体性别鉴定的影响。
J Assist Reprod Genet. 1996 Aug;13(7):586-91. doi: 10.1007/BF02066613.
7
Reliable sex determination of mouse preimplantation embryos by PCR amplification of male-specific genes in single blastomeres.通过对单个卵裂球中雄性特异性基因进行PCR扩增来可靠地鉴定小鼠植入前胚胎的性别。
J Med Assoc Thai. 1996 Dec;79 Suppl 1:S78-87.
8
Enzymatic amplification of specific deoxyribonucleic acid sequences from single cells: evaluation of a simplified and rapid method for use in preimplantation genetic diagnosis.单细胞特异性脱氧核糖核酸序列的酶促扩增：一种用于植入前基因诊断的简化快速方法的评估
Fertil Steril. 1995 Aug;64(2):255-60. doi: 10.1016/s0015-0282(16)57719-x.
9
Sex determination by simultaneous application of polymerase chain reaction and fluorescent in situ hybridization on the same blastomere of a pre-embryo.通过在同一胚胎前体的卵裂球上同时应用聚合酶链反应和荧光原位杂交进行性别鉴定。
Fertil Steril. 1996 Sep;66(3):490-2. doi: 10.1016/s0015-0282(16)58527-6.
10
Gender determination by multiplex PCR amplification of alphoid repeat sequences from single cells.通过对单细胞中的α卫星重复序列进行多重PCR扩增来确定性别。
Biotechniques. 1994 Jan;16(1):82-4.

引用本文的文献

1
Gender selection: cultural and religious perspectives.性别选择：文化与宗教视角
J Assist Reprod Genet. 2002 Sep;19(9):400-10. doi: 10.1023/a:1016807605886.
2
An accurate and rapid gender determination assay in single cells by the capillary polymerase chain reaction method.采用毛细管聚合酶链反应法对单细胞进行准确快速的性别鉴定分析。
J Assist Reprod Genet. 1999 Nov;16(10):551-4. doi: 10.1023/a:1020505406143.
3
Review: preimplantation diagnosis of inherited disease.
J Inherit Metab Dis. 1996;19(6):709-23. doi: 10.1007/BF01799159.

本文引用的文献

1
A human Y-chromosome specific repeated DNA family (DYZ1) consists of a tandem array of pentanucleotides.
Nucleic Acids Res. 1986 Oct 10;14(19):7569-80. doi: 10.1093/nar/14.19.7569.
2
An improved method for prenatal diagnosis of genetic diseases by analysis of amplified DNA sequences. Application to hemophilia A.一种通过分析扩增的DNA序列进行遗传性疾病产前诊断的改进方法。应用于甲型血友病。
N Engl J Med. 1987 Oct 15;317(16):985-90. doi: 10.1056/NEJM198710153171603.
3
Detection of sickle cell anaemia and thalassaemias.镰状细胞贫血和地中海贫血的检测。
4
Preimplantation diagnosis of inherited disease by embryo biopsy: an update of the world figures.
J Assist Reprod Genet. 1996 Feb;13(2):90-5. doi: 10.1007/BF02072527.
5
Identifying the sex of human preimplantation embryos in X-linked disease: amplification efficiency of a Y-specific alphoid repeat from single blastomeres with two lysis protocols.在X连锁疾病中鉴定人类植入前胚胎的性别：两种裂解方案对单个卵裂球中Y特异性α卫星重复序列的扩增效率
J Assist Reprod Genet. 1996 Feb;13(2):125-32. doi: 10.1007/BF02072533.
6
A fast and efficient method for simultaneous X and Y in situ hybridization of human blastomeres.一种用于人类卵裂球X和Y同时原位杂交的快速高效方法。
J Assist Reprod Genet. 1993 Jan;10(1):82-90. doi: 10.1007/BF01204446.
7
Sex determination in single mouse blastomeres by polymerase chain reaction.通过聚合酶链反应对单个小鼠卵裂球进行性别鉴定。
J Assist Reprod Genet. 1993 Feb;10(2):151-6. doi: 10.1007/BF01207739.
8
Preimplantation diagnosis.植入前诊断。
West J Med. 1993 Sep;159(3):301-7.
9
Chromosome abnormalities in human arrested preimplantation embryos: a multiple-probe FISH study.人类植入前停滞胚胎中的染色体异常：一项多探针荧光原位杂交研究。
Am J Hum Genet. 1994 Jul;55(1):150-9.
10
Optimal polymerase chain reaction amplification for preimplantation diagnosis in cystic fibrosis (delta F508).用于囊性纤维化（ΔF508）植入前诊断的最佳聚合酶链反应扩增
BMJ. 1995 Aug 26;311(7004):536-40. doi: 10.1136/bmj.311.7004.536.
Nature. 1987;329(6137):293-4. doi: 10.1038/329293b0.
4
Amplification and analysis of DNA sequences in single human sperm and diploid cells.单个人类精子和二倍体细胞中DNA序列的扩增与分析。
Nature. 1988 Sep 29;335(6189):414-7. doi: 10.1038/335414a0.
5
Diagnosis of sickle cell anemia and beta-thalassemia with enzymatically amplified DNA and nonradioactive allele-specific oligonucleotide probes.利用酶促扩增DNA和非放射性等位基因特异性寡核苷酸探针诊断镰状细胞贫血和β地中海贫血。
N Engl J Med. 1988 Sep 1;319(9):537-41. doi: 10.1056/NEJM198809013190903.
6
Splice junction mutation in some Ashkenazi Jews with Tay-Sachs disease: evidence against a single defect within this ethnic group.一些患有泰-萨克斯病的阿什肯纳兹犹太人存在剪接连接突变：这一证据反驳了该族群存在单一缺陷的观点。
Proc Natl Acad Sci U S A. 1988 Jun;85(11):3955-9. doi: 10.1073/pnas.85.11.3955.
7
Prospects for prenatal diagnosis during preimplantation human development.
Baillieres Clin Obstet Gynaecol. 1987 Sep;1(3):747-64. doi: 10.1016/s0950-3552(87)80015-9.
8
Rapid DNA analysis of alpha 1-antitrypsin deficiency: application of an improved method for amplifying mutated gene sequences.
Lab Invest. 1988 Sep;59(3):403-8.
9
Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.用于镰状细胞贫血诊断的β-珠蛋白基因组序列的酶促扩增及限制性酶切位点分析。
Science. 1985 Dec 20;230(4732):1350-4. doi: 10.1126/science.2999980.
10
Chromosome-specific alpha satellite DNA: nucleotide sequence analysis of the 2.0 kilobasepair repeat from the human X chromosome.染色体特异性α卫星DNA：来自人类X染色体的2.0千碱基对重复序列的核苷酸序列分析
Nucleic Acids Res. 1985 Apr 25;13(8):2731-43. doi: 10.1093/nar/13.8.2731.