Le Thuc T, Rehrer Charles W, Huff Terry B, Nichols Maxine B, Camarillo Ignacio G, Cheng Ji-Xin
Weldon School of Biomedical Engineering, Department of Biological Sciences, Purdue Cancer Center, and Purdue Oncological Sciences Center, Purdue University, West Lafayette, IN 47907, USA.
Mol Imaging. 2007 May-Jun;6(3):205-11.
Obesity is an established risk factor for breast cancer incidence and mortality. However, the mechanism that links obesity to tumorigenesis is not well understood. Here we combined nonlinear optical imaging technologies with an early-onset diet-induced obesity breast cancer animal model to evaluate the impact of obesity on the composition of mammary gland and tumor stroma. Using coherent anti-Stokes Raman scattering and second harmonic generation on the same platform, we simultaneously imaged mammary adipocytes, blood capillaries, collagen fibrils, and tumor cells without any labeling. We observed that obesity increases the size of lipid droplets of adipocytes in mammary gland and collagen content in mammary tumor stroma, respectively. Such impacts of obesity on mammary gland and tumor stroma could not be analyzed using standard two-dimensional histologic evaluation. Given the importance of mammary stroma to the growth and migration of tumor cells, our observation provides the first imaging evidence that supports the relationship between obesity and breast cancer risk.
肥胖是乳腺癌发病和死亡的既定风险因素。然而，将肥胖与肿瘤发生联系起来的机制尚未完全明确。在此，我们将非线性光学成像技术与早发性饮食诱导肥胖乳腺癌动物模型相结合，以评估肥胖对乳腺和肿瘤基质组成的影响。利用同一平台上的相干反斯托克斯拉曼散射和二次谐波产生技术，我们在无需任何标记的情况下，同时对乳腺脂肪细胞、毛细血管、胶原纤维和肿瘤细胞进行了成像。我们观察到，肥胖分别增加了乳腺中脂肪细胞的脂滴大小和乳腺肿瘤基质中的胶原含量。肥胖对乳腺和肿瘤基质的这种影响无法通过标准的二维组织学评估进行分析。鉴于乳腺基质对肿瘤细胞生长和迁移的重要性，我们的观察提供了首个成像证据，支持肥胖与乳腺癌风险之间的关系。
