Tufts University, Department of Biomedical Engineering, Medford, Massachusetts, United States.
Giresun University, Department of Biology, Giresun, Turkey.
J Biomed Opt. 2020 Jan;25(1):1-14. doi: 10.1117/1.JBO.25.1.014513.
Obesity is associated with a higher risk of developing breast cancer and with worse disease outcomes for women of all ages. The composition, density, and organization of the breast tissue stroma are also known to play an important role in the development and progression of the disease. However, the connections between obesity and stromal remodeling are not well understood. We sought to characterize detailed organization features of the collagen matrix within healthy and cancerous breast tissues acquired from mice exposed to either a normal or high fat (obesity inducing) diet. We performed second-harmonic generation and spectral two-photon excited fluorescence imaging, and we extracted the level of collagen-associated fluorescence (CAF) along with metrics of collagen content, three-dimensional, and two-dimensional organization. There were significant differences in the CAF intensity and overall collagen organization between normal and tumor tissues; however, obesity-enhanced changes in these metrics, especially when three-dimensional organization metrics were considered. Thus, our studies indicate that obesity impacts significantly collagen organization and structure and the related pathways of communication may be important future therapeutic targets.
肥胖与乳腺癌发病风险增加以及各年龄段女性疾病预后恶化相关。乳腺组织基质的组成、密度和组织也被认为在疾病的发生和发展中发挥着重要作用。然而，肥胖与基质重构之间的联系尚不清楚。我们试图描述从暴露于正常或高脂肪（诱导肥胖）饮食的小鼠中获得的健康和癌组织内胶原基质的详细组织特征。我们进行了二次谐波产生和光谱双光子激发荧光成像，并提取了与胶原相关的荧光（CAF）水平以及胶原含量、三维和二维组织的度量。正常组织和肿瘤组织之间的 CAF 强度和整体胶原组织存在显著差异；然而，肥胖增强了这些指标的变化，特别是当考虑三维组织指标时。因此，我们的研究表明，肥胖显著影响胶原组织和结构，相关的信号通路可能是未来重要的治疗靶点。
