Department of Biology, Faculty of Science and Letters, Adiyaman University, 02030, Adiyaman, Turkey,
Cytotechnology. 2012 Jan;64(1):83-94. doi: 10.1007/s10616-011-9396-z. Epub 2011 Oct 9.
The genotoxic and anti-genotoxic effects of Stachys petrokosmos leaf extracts (Sp) were investigated in human lymphocytes. The cells were treated with 1.5, 3.0 and 6.0 μL/mL concentrations of Sp leaf extracts for 24 and 48 h treatment periods in the absence and presence of metabolic activator (S9mix). In the absence of S9mix, Sp alone did not induce chromosome aberrations and formation of micronucleus while inducing the mean sister chromatid exchange at the highest concentration. In addition, Sp decreased the mutagenic effect of mitomycin-c. Sp alone showed a cytotoxic effect determined by a decrease in the proliferation index, mitotic index and nuclear division index. On the other hand a mixture of Sp and mitomycin-c resulted in a higher cytotoxic effect especially for 48 h treatment period. In the presence of S9mix, Sp was not genotoxic and cytotoxic however, it showed an anti-genotoxic effect by decreasing the effects of cyclophosphamide.
研究了野芝麻叶提取物（Sp）对人淋巴细胞的遗传毒性和抗遗传毒性作用。将细胞用 1.5、3.0 和 6.0 μL/mL 的 Sp 叶提取物浓度处理 24 和 48 h 处理期，在无代谢激活剂（S9mix）和有代谢激活剂的情况下。在没有 S9mix 的情况下，Sp 单独处理不会诱导染色体畸变和微核形成，而在最高浓度下诱导平均姐妹染色单体交换。此外，Sp 降低了丝裂霉素 C 的诱变作用。Sp 单独处理表现出细胞毒性作用，表现为增殖指数、有丝分裂指数和核分裂指数下降。另一方面，Sp 和丝裂霉素 C 的混合物在 48 h 处理期表现出更高的细胞毒性作用。在有 S9mix 的情况下，Sp 没有遗传毒性和细胞毒性，但通过降低环磷酰胺的作用表现出抗遗传毒性作用。
