Ates Ozkan, Cayli Suleyman, Gurses Iclal, Yucel Neslihan, Altinoz Eyup, Iraz Mustafa, Kocak Ayhan, Yologlu Saim
Department of Neurosurgery, School of Medicine, Inonu University, Malatya, Turkey.
Neurol Res. 2007 Sep;29(6):533-9. doi: 10.1179/016164107X172121.
Previous reports documented demonstrated that melatonin, a free radical scavenger, is important in protecting against oxidative stress-induced tissue damage after spinal cord injury (SCI). This study was undertaken to investigate the effects of pinealectomy (PX) and administration of exogenous melatonin after SCI in rats. These animals were randomized into six groups, each having 12 rats. Group 1 underwent laminectomy alone. Group 2 underwent laminectomy followed by SCI and received no medication. Group 3 underwent laminectomy followed by SCI and received melatonin. Group 4 underwent PX and laminectomy alone. Group 5 underwent PX and laminectomy followed by SCI and received no medication. Group 6 underwent PX and laminectomy followed by SCI and received melatonin. Melatonin (100 mg/kg) was given intraperitoneally immediately after trauma to the rats in the groups 3 and 6. PX caused a significant increase in the malondialdehyde (MDA), nitrite oxide (NO), glutathione (GSH), xanthine oxidase (XO) levels and decrease in GSH levels as compared with the control group. Trauma to the spinal cord results in significantly higher oxidative stress. Melatonin administration significantly reduced MDA, XO and NO levels, and increased GSH levels in the spinal cord after trauma. Exogenous melatonin treatment after trauma attenuated tissue lesion area and accelerated motor recovery rate. These findings suggest that reduction in endogenous melatonin after PX makes the rats more vulnerable to trauma and exogenous melatonin administration has an important neuroprotective effect on the level of the spinal cord.
先前的报告表明，褪黑素作为一种自由基清除剂，在预防脊髓损伤（SCI）后氧化应激诱导的组织损伤方面具有重要作用。本研究旨在探讨松果体切除（PX）及SCI后给予外源性褪黑素对大鼠的影响。将这些动物随机分为六组，每组12只大鼠。第1组仅接受椎板切除术。第2组接受椎板切除术后再行SCI，且未接受药物治疗。第3组接受椎板切除术后再行SCI，并接受褪黑素治疗。第4组仅接受PX和椎板切除术。第5组接受PX和椎板切除术后再行SCI，且未接受药物治疗。第6组接受PX和椎板切除术后再行SCI，并接受褪黑素治疗。第3组和第6组大鼠在创伤后立即腹腔注射褪黑素（100 mg/kg）。与对照组相比，PX导致丙二醛（MDA）、一氧化氮（NO）、谷胱甘肽（GSH）、黄嘌呤氧化酶（XO）水平显著升高，GSH水平降低。脊髓创伤导致氧化应激显著升高。给予褪黑素可显著降低创伤后脊髓中MDA、XO和NO水平，并提高GSH水平。创伤后给予外源性褪黑素可减轻组织损伤面积，加快运动恢复速度。这些研究结果表明，PX后内源性褪黑素减少使大鼠更容易受到创伤，而给予外源性褪黑素对脊髓水平具有重要的神经保护作用。
