[DNA修复基因XRCC1、XRCC3基因多态性与慢性苯中毒易感性的关系]

[Association between genetic polymorphisms of DNA repair genes XRCC1, XRCC3 and susceptibility to chronic benzene poisoning].

作者信息

Huang Hui-long, Xu Jian-ning, Wang Quan-kai, Yang Min, Wang Ya-wen, Chen Yan, Li Gui-lan

机构信息

National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.

出版信息

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2007 Apr;25(4):193-6.

PMID:17535647

Abstract

OBJECTIVE

To explore the association between genetic polymorphisms of DNA repair genes XRCC1, XRCC3 and susceptibility to chronic benzene poisoning.

METHODS

A case-control study was conducted. Eighty patients with chronic benzene poisoning and 62 workers occupationally exposed to benzene who were engaged in the same working time and job title as patients were investigated. Polymerase chain reaction-restriction fragments length polymorphism (PCR-RFLP) was used to detect the single nucleotide polymorphisms on C26304T, G27466A, G28152A, G36189A of XRCC1 and C18067T of XRCC3. The relationship between them and latency of chronic benzene poisoning was analyzed by Kaplan-Meier method.

RESULTS

A correlation for XRCC3 18067C/T compared with C/C genotype was found (OR=0.233, 95% CI 0.085 approximately 0.639, P=0.0046). Patients who were XRCC1 27466G/G homozygous wild genotype developed chronic benzene poisoning average 6 years later than those had homozygous (27466A/A) or heterozygous (27466G/A) mutant alleles.

CONCLUSION

Subjects with XRCC3 18067T variant allele are tolerance sub-group to benzene poisoning. Patients carrying XRCC1 27466 G/G genotype develop chronic benzene poisoning later.

摘要

目的

探讨DNA修复基因XRCC1、XRCC3的基因多态性与慢性苯中毒易感性之间的关系。

方法

进行病例对照研究。调查了80例慢性苯中毒患者和62名与患者工作时间和职称相同的职业性接触苯的工人。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）法检测XRCC1基因C26304T、G27466A、G28152A、G36189A位点及XRCC3基因C18067T位点的单核苷酸多态性。采用Kaplan-Meier法分析它们与慢性苯中毒潜伏期的关系。