Xiang Yong, Huang Yuemin, Xiong Lizhong
National Center of Plant Gene Research, National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China.
Plant Physiol. 2007 Jul;144(3):1416-28. doi: 10.1104/pp.107.101295. Epub 2007 May 25.
Plants respond to adverse environments by initiating a series of signaling processes that often involves diverse protein kinases, including calcineurin B-like protein-interacting protein kinases (CIPKs). In this study, putative CIPK genes (OsCIPK01-OsCIPK30) in the rice (Oryza sativa) genome were surveyed for their transcriptional responses to various abiotic stresses. The results showed that 20 OsCIPK genes were differentially induced by at least one of the stresses, including drought, salinity, cold, polyethylene glycol, and abscisic acid treatment. Most of the genes induced by drought or salt stress were also induced by abscisic acid treatment but not by cold. A few CIPK genes containing none of the reported stress-responsive cis-elements in their promoter regions were also induced by multiple stresses. To prove that some of these stress-responsive OsCIPK genes are potentially useful for stress-tolerance improvement, three CIPK genes (OsCIPK03, OsCIPK12, and OsCIPK15) were overexpressed in japonica rice 'Zhonghua 11'. Transgenic plants overexpressing the transgenes OsCIPK03, OsCIPK12, and OsCIPK15 showed significantly improved tolerance to cold, drought, and salt stress, respectively. Under cold and drought stresses, OsCIPK03- and OsCIPK12-overexpressing transgenic plants accumulated significantly higher contents of proline and soluble sugars than the wild type. Putative proline synthetase and transporter genes had significantly higher expression level in the transgenic plants than in the wild type. The differentially induced expression of OsCIPK genes by different stresses and the examples of improved stress tolerance of the OsCIPK transgenic rice suggest that rice CIPK genes have diverse roles in different stress responses and some of them may possess potential usefulness in stress-tolerance improvement of rice.
植物通过启动一系列信号传导过程来应对不利环境，这些过程通常涉及多种蛋白激酶，包括钙调神经磷酸酶B类蛋白相互作用蛋白激酶（CIPK）。在本研究中，对水稻（Oryza sativa）基因组中的假定CIPK基因（OsCIPK01 - OsCIPK30）进行了调查，以了解它们对各种非生物胁迫的转录反应。结果表明，20个OsCIPK基因受到至少一种胁迫的差异诱导，这些胁迫包括干旱、盐度、寒冷、聚乙二醇和脱落酸处理。大多数受干旱或盐胁迫诱导的基因也受脱落酸处理诱导，但不受寒冷诱导。一些在其启动子区域不含已报道的胁迫响应顺式元件的CIPK基因也受到多种胁迫的诱导。为了证明其中一些胁迫响应性OsCIPK基因可能有助于提高胁迫耐受性，在粳稻‘中华11’中过表达了三个CIPK基因（OsCIPK03、OsCIPK12和OsCIPK15）。过表达转基因OsCIPK03、OsCIPK12和OsCIPK15的转基因植物分别对寒冷、干旱和盐胁迫表现出显著提高的耐受性。在寒冷和干旱胁迫下，过表达OsCIPK03和OsCIPK12的转基因植物积累的脯氨酸和可溶性糖含量明显高于野生型。假定的脯氨酸合成酶和转运蛋白基因在转基因植物中的表达水平明显高于野生型。不同胁迫对OsCIPK基因的差异诱导表达以及OsCIPK转基因水稻胁迫耐受性提高的实例表明，水稻CIPK基因在不同的胁迫反应中具有多种作用，其中一些基因可能在提高水稻胁迫耐受性方面具有潜在用途。
