Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun, 130033, China.
Plant Cell Rep. 2023 Aug;42(8):1291-1310. doi: 10.1007/s00299-023-03031-8. Epub 2023 May 17.
The salt-tolerance of transgenic soybean cleared for environmental release was improved by stable over-expression of AhBADH gene from Atriplex hortensis, which was demonstrated through molecular analysis and field experiments. An effective strategy for increasing the productivity of major crops under salt stress conditions is the development of transgenics that harbor genes responsible for salinity tolerance. Betaine aldehyde dehydrogenase (BADH) is a key enzyme involved in the biosynthesis of the osmoprotectant, glycine betaine (GB), and osmotic balance in plants, and several plants transformed with BADH gene have shown significant improvements in salt tolerance. However, very few field-tested transgenic cultivars have been reported, as most of the transgenic studies are limited to laboratory or green house experiments. In this study, we demonstrated through field experiments that AhBADH from Atriplex hortensis confers salt tolerance when transformed into soybean (Glycine max L.). AhBADH was successfully introduced into soybean by Agrobacterium mediated transformation. A total of 256 transgenic plants were obtained, out of which 47 lines showed significant enhancement of salt tolerance compared to non-transgenic control plants. Molecular analyses of the transgenic line TL2 and TL7 with the highest salt tolerance exhibited stable inheritance and expression of AhBADH in progenies with a single copy insertion. TL1, TL2 and TL7 exhibited stable enhanced salt tolerance and improved agronomic traits when subjected to 300mM NaCl treatment. Currently, the transgenic line TL2 and TL7 with stable enhanced salt tolerance, which have been cleared for environmental release, are under biosafety assessment. TL 2 and TL7 stably expressing AhBADH could then be applied in commercial breeding experiments to genetically improve salt tolerance in soybean.
耐盐转基因大豆的开发是提高作物在盐胁迫条件下生产力的有效策略，该策略通过稳定表达来自滨藜的 AhBADH 基因来提高耐盐性，这一策略已通过分子分析和田间试验得到证实。甜菜醛脱氢酶（BADH）是参与合成渗透调节剂甘氨酸甜菜碱（GB）和植物渗透平衡的关键酶，已转化 BADH 基因的几种植物表现出显著的耐盐性提高。然而，由于大多数转基因研究仅限于实验室或温室实验，因此报道的田间试验转基因品种非常少。在这项研究中，我们通过田间试验表明，来自滨藜的 AhBADH 在转化为大豆（Glycine max L.）时赋予其耐盐性。AhBADH 通过农杆菌介导的转化成功引入大豆。共获得 256 株转基因植株，其中 47 株与非转基因对照植株相比表现出显著的耐盐性增强。对具有最高耐盐性的转基因株系 TL2 和 TL7 的分子分析表明，AhBADH 在具有单拷贝插入的后代中稳定遗传和表达。TL1、TL2 和 TL7 在 300mM NaCl 处理下表现出稳定增强的耐盐性和改良的农艺性状。目前，具有稳定增强耐盐性且已获准环境释放的转基因株系 TL2 和 TL7 正在进行生物安全评估。然后，可以将稳定表达 AhBADH 的 TL2 和 TL7 转基因株系应用于商业育种实验中，以遗传改良大豆的耐盐性。
