特定序列脱氧核糖寡核苷酸的酶促合成。脱氧核糖寡核苷酸合成。
Enzymatic synthesis of deoxyribo-oligonucleotides of defined sequence. Deoxyribo-oligonucleotide synthesis.
作者信息
Gillam S, Waterman K, Doel M, Smith M
出版信息
Nucleic Acids Res. 1974 Dec;1(12):1649-64. doi: 10.1093/nar/1.12.1649.
Abstract
An enzyme, which is probably identical with polynucleotide phosphorylase, was prepared from Escherichiacoli B. In the presence of Mn(2+) it catalyzes the addition of one (and to a slight extent more) residue of deoxyribonucleotide residue from the diphosphate to an oligodeoxyribonucleotide primer. The shortest effective primers contained three phosphate residues. Ribodinucleotides were effective as primers and accepted two or three deoxyribonucleotide residues under these conditions. The application of the procedures to the convenient synthesis of certain defined oligodeoxyribonucleotides up to nine residues long is discussed.
摘要
从大肠杆菌B中制备了一种酶，它可能与多核苷酸磷酸化酶相同。在锰离子存在的情况下，它催化将一个（在一定程度上更多）脱氧核糖核苷酸残基从二磷酸添加到寡脱氧核糖核苷酸引物上。最短的有效引物含有三个磷酸残基。核糖二核苷酸作为引物是有效的，并且在这些条件下可以接受两到三个脱氧核糖核苷酸残基。讨论了将该方法应用于方便地合成某些确定的长达九个残基的寡脱氧核糖核苷酸。
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