特定序列寡聚脱氧核糖核苷酸的酶促合成。多核苷酸磷酸化酶催化将脱氧核糖核苷酸添加到良好或不良受体的引物上。
Enzymatic synthesis of oligodeoxyribonucleotides of defined sequence. Polynucleotide phosphorylase catalysed addition of deoxyribonucleotides to primers which are good or poor acceptors.
作者信息
Trip E M, Smith M
出版信息
Nucleic Acids Res. 1978 May;5(5):1529-38. doi: 10.1093/nar/5.5.1529.
Abstract
Analytical high-pressure anion-exchange chromatography on RPC-5 has been used to study the behaviour of a good primer, d(pT-T-A-G), and a poor primer, d(pT-T-T-T-T-T) in the E. coli polynucleotide phosphorylase-catalysed reactions of dADP, dCDP, dGDP and dTDP where the primer is extended, predominantly, by one or two nucleotides. The experiments provide some generalizations for obtaining optimal yields in preparative reactions. In the course of the experiments, examples of anomalous behaviour of oligonucleotides on RPC-5 were encountered and these are discussed.
摘要
采用RPC - 5进行分析型高压阴离子交换色谱法，研究了良好引物d(pT - T - A - G)和不良引物d(pT - T - T - T - T - T)在大肠杆菌多核苷酸磷酸化酶催化的dADP、dCDP、dGDP和dTDP反应中的行为，在这些反应中引物主要延伸一或两个核苷酸。这些实验为在制备反应中获得最佳产率提供了一些一般性结论。在实验过程中，遇到了寡核苷酸在RPC - 5上的异常行为实例，并对此进行了讨论。
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Enzymatic synthesis of oligodeoxyribonucleotides of defined sequence. Polynucleotide phosphorylase catalysed addition of deoxyribonucleotides to primers which are good or poor acceptors.特定序列寡聚脱氧核糖核苷酸的酶促合成。多核苷酸磷酸化酶催化将脱氧核糖核苷酸添加到良好或不良受体的引物上。
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本文引用的文献
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Improved separation of transfer RNA's on polychlorotrifuoroethylene-supported reversed-phase chromatography columns.在聚氯三氟乙烯支持的反相色谱柱上改进转运RNA的分离。
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Enzymatic synthesis of deoxyribo-oligonucleotides of defined sequence. Deoxyribo-oligonucleotide synthesis.特定序列脱氧核糖寡核苷酸的酶促合成。脱氧核糖寡核苷酸合成。
Nucleic Acids Res. 1974 Dec;1(12):1649-64. doi: 10.1093/nar/1.12.1649.
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Enzymatic synthesis of deoxyribo-oligonucleotides of defined sequence. Properties of the enzyme.特定序列脱氧核糖寡核苷酸的酶促合成。该酶的性质。
Nucleic Acids Res. 1974 Dec;1(12):1631-47. doi: 10.1093/nar/1.12.1631.
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Enzymatic synthesis of deoxyribo-oligonucleotides of defined sequence.
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Anion-exchange chromatography on reversed-phase columns: isolation and assay of nucleosides, nucleotides, and oligonucleotides from nucleic acids and from cytoplasm.反相柱上的阴离子交换色谱法：从核酸和细胞质中分离和测定核苷、核苷酸及寡核苷酸。
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"Single Addition" substrates for the synthesis of specific oligoribonucleotides with polynucleotide phosphorylase. Synthesis of 2'-(alpha-methoxyethy) nucleoside 5'-diphosphates.用于通过多核苷酸磷酸化酶合成特定寡核糖核苷酸的“单次添加”底物。2'-（α-甲氧基乙基）核苷5'-二磷酸的合成。
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The base-pairing specificity of cellulose-pdT9.纤维素-pdT9的碱基配对特异性
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