通过优化的实时聚合酶链反应方法对人呼吸道样本中的肺炎支原体进行灵敏检测。
Sensitive detection of Mycoplasma pneumoniae in human respiratory tract samples by optimized real-time PCR approach.
作者信息
Dumke Roger, Schurwanz Nicol, Lenz Matthias, Schuppler Markus, Lück Christian, Jacobs Enno
机构信息
Technical University Dresden, Medical Faculty Carl Gustav Carus, Institute of Medical Microbiology and Hygiene, Fetscherstrasse 74, D-01307 Dresden, Germany.
出版信息
J Clin Microbiol. 2007 Aug;45(8):2726-30. doi: 10.1128/JCM.00321-07. Epub 2007 May 30.
Abstract
To enhance the sensitivity of the available real-time PCR systems for the detection of Mycoplasma pneumoniae, we established a method to amplify copies of the repetitive element repMp1. In a study of respiratory tract samples, we found that, compared to the use of the conserved part of the P1 adhesin gene as a monocopy target, the use of the repMp1-PCR showed an increase in the detected genome equivalents by a factor of 22.
摘要
为提高现有实时PCR系统检测肺炎支原体的灵敏度，我们建立了一种扩增重复元件repMp1拷贝的方法。在一项呼吸道样本研究中，我们发现，与使用P1黏附素基因的保守部分作为单拷贝靶标相比，使用repMp1-PCR检测到的基因组当量增加了22倍。
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本文引用的文献
1
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J Clin Microbiol. 2006 Jul;44(7):2567-70. doi: 10.1128/JCM.00495-06.
2
Real-time detection of Mycoplasma pneumoniae in respiratory samples with an internal processing control.采用内部处理对照对呼吸道样本中的肺炎支原体进行实时检测。
J Med Microbiol. 2006 Feb;55(Pt 2):149-155. doi: 10.1099/jmm.0.46281-0.
3
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Int J Med Microbiol. 2005 Aug;295(4):279-85. doi: 10.1016/j.ijmm.2005.05.001.
4
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Diagn Microbiol Infect Dis. 2005 May;52(1):7-14. doi: 10.1016/j.diagmicrobio.2005.01.005.
5
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