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通过优化的实时聚合酶链反应方法对人呼吸道样本中的肺炎支原体进行灵敏检测。

Sensitive detection of Mycoplasma pneumoniae in human respiratory tract samples by optimized real-time PCR approach.

作者信息

Dumke Roger, Schurwanz Nicol, Lenz Matthias, Schuppler Markus, Lück Christian, Jacobs Enno

机构信息

Technical University Dresden, Medical Faculty Carl Gustav Carus, Institute of Medical Microbiology and Hygiene, Fetscherstrasse 74, D-01307 Dresden, Germany.

出版信息

J Clin Microbiol. 2007 Aug;45(8):2726-30. doi: 10.1128/JCM.00321-07. Epub 2007 May 30.

DOI:10.1128/JCM.00321-07
PMID:17537933
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1951254/
Abstract

To enhance the sensitivity of the available real-time PCR systems for the detection of Mycoplasma pneumoniae, we established a method to amplify copies of the repetitive element repMp1. In a study of respiratory tract samples, we found that, compared to the use of the conserved part of the P1 adhesin gene as a monocopy target, the use of the repMp1-PCR showed an increase in the detected genome equivalents by a factor of 22.

摘要

为提高现有实时PCR系统检测肺炎支原体的灵敏度，我们建立了一种扩增重复元件repMp1拷贝的方法。在一项呼吸道样本研究中，我们发现，与使用P1黏附素基因的保守部分作为单拷贝靶标相比，使用repMp1-PCR检测到的基因组当量增加了22倍。

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本文引用的文献

1
Culture-independent molecular subtyping of Mycoplasma pneumoniae in clinical samples.临床样本中肺炎支原体的非培养分子分型
J Clin Microbiol. 2006 Jul;44(7):2567-70. doi: 10.1128/JCM.00495-06.
2
Real-time detection of Mycoplasma pneumoniae in respiratory samples with an internal processing control.采用内部处理对照对呼吸道样本中的肺炎支原体进行实时检测。
J Med Microbiol. 2006 Feb;55(Pt 2):149-155. doi: 10.1099/jmm.0.46281-0.
3
Detection of Mycoplasma pneumoniae in serum specimens from patients with mycoplasma pneumonia by PCR.采用聚合酶链反应（PCR）检测支原体肺炎患者血清标本中的肺炎支原体。
Int J Med Microbiol. 2005 Aug;295(4):279-85. doi: 10.1016/j.ijmm.2005.05.001.
4
Chlamydia pneumoniae and Mycoplasma pneumoniae in young children from China with community-acquired pneumonia.中国社区获得性肺炎幼儿中的肺炎衣原体和肺炎支原体
Diagn Microbiol Infect Dis. 2005 May;52(1):7-14. doi: 10.1016/j.diagmicrobio.2005.01.005.
5
The pneumoplex assays, a multiplex PCR-enzyme hybridization assay that allows simultaneous detection of five organisms, Mycoplasma pneumoniae, Chlamydia (Chlamydophila) pneumoniae, Legionella pneumophila, Legionella micdadei, and Bordetella pertussis, and its real-time counterpart.肺炎病原体检测，一种多重聚合酶链反应-酶杂交检测法，可同时检测五种病原体，即肺炎支原体、肺炎衣原体（嗜衣原体）、嗜肺军团菌、麦氏军团菌和百日咳博德特氏菌，以及其实时检测对应方法。
J Clin Microbiol. 2005 Feb;43(2):565-71. doi: 10.1128/JCM.43.2.565-571.2005.
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