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血液样本处理延迟会影响血培养阳性时间以及革兰氏染色-吖啶橙白细胞甩片试验的结果。

Delayed processing of blood samples influences time to positivity of blood cultures and results of Gram stain-acridine orange leukocyte Cytospin test.

作者信息

Schwetz I, Hinrichs G, Reisinger E C, Krejs G J, Olschewski H, Krause R

机构信息

Division of Infectious Diseases, Department of Pulmonology, Medical University of Graz, Graz, Austria.

出版信息

J Clin Microbiol. 2007 Aug;45(8):2691-4. doi: 10.1128/JCM.00085-07. Epub 2007 May 30.

DOI:10.1128/JCM.00085-07
PMID:17537945
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1951239/
Abstract

We investigated in vitro whether storage of blood samples influences the time to positivity used for the calculation of the differential time to positivity (DTP) and the results of the Gram stain-acridine orange leukocyte Cytospin (AOLC) test. A 24-hour storage of blood samples at room temperature may lead to false-negative DTP and false-positive Gram stain-AOLC test results, whereas storage at 4 degrees C does not.

摘要

我们在体外研究了血样储存是否会影响用于计算阳性差异时间（DTP）的阳性时间以及革兰氏染色-吖啶橙白细胞甩片（AOLC）试验的结果。血样在室温下储存24小时可能会导致DTP出现假阴性以及革兰氏染色-AOLC试验结果出现假阳性，而在4℃下储存则不会。

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Delayed processing of blood samples influences time to positivity of blood cultures and results of Gram stain-acridine orange leukocyte Cytospin test.血液样本处理延迟会影响血培养阳性时间以及革兰氏染色-吖啶橙白细胞甩片试验的结果。
J Clin Microbiol. 2007 Aug;45(8):2691-4. doi: 10.1128/JCM.00085-07. Epub 2007 May 30.
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本文引用的文献

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Differential time to positivity: a useful method for diagnosing catheter-related bloodstream infections.阳性时间差异：一种诊断导管相关血流感染的有用方法。
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Bloodstream infection in neutropenic cancer patients related to short-term nontunnelled catheters determined by quantitative blood cultures, differential time to positivity, and molecular epidemiological typing with pulsed-field gel electrophoresis.通过定量血培养、阳性时间差异以及脉冲场凝胶电泳分子流行病学分型确定的中性粒细胞减少癌症患者与短期非隧道式导管相关的血流感染
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Intravascular catheter-associated infections.血管内导管相关感染
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5
Diagnosis of catheter-related bacteraemia: a prospective comparison of the time to positivity of hub-blood versus peripheral-blood cultures.导管相关菌血症的诊断：中心静脉血与外周血培养阳性时间的前瞻性比较
Lancet. 1999 Sep 25;354(9184):1071-7. doi: 10.1016/s0140-6736(98)11134-0.
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Quantitative culture of intravenous catheters and other intravascular inserts.静脉导管及其他血管内植入物的定量培养
J Infect Dis. 1980 Jun;141(6):781-6. doi: 10.1093/infdis/141.6.781.
7
Association between microorganism growth at the catheter insertion site and colonization of the catheter in patients receiving total parenteral nutrition.接受全胃肠外营养患者的导管插入部位微生物生长与导管定植之间的关联。
Surgery. 1982 Oct;92(4):720-7.
8
Diagnosis of central venous catheter-related sepsis. Critical level of quantitative tip cultures.中心静脉导管相关败血症的诊断。定量尖端培养的临界水平。
Arch Intern Med. 1987 May;147(5):873-7.
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Quantitative tip culture methods and the diagnosis of central venous catheter-related infections.定量尖端培养方法与中心静脉导管相关感染的诊断
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