Goi Nobuhiro, Hirai Yuuko, Harada Hitoshi, Ikari Akira, Ono Takahiko, Kinae Naohide, Hiramatsu Mitsuo, Nakamura Kimitsugu, Takagi Kuniaki
Department of Environmental Biochemistry and Toxicology, University of Shizuoka School of Pharmaceutical Science, Yada, Shizuoka, Japan.
J Toxicol Sci. 2007 May;32(2):121-7. doi: 10.2131/jts.32.121.
Saliva is the first body fluid to encounter exogenous materials or gases such as cigarette smoke (CS). The aim of this study was to examine whether smoking affects oral peroxidase (OPO) reactivity to mental stress. The subjects were 39 non-smokers and 10 smokers. In the experiment, the Kraepelin psychodiagnostic test as a psychological stressor and saliva was sampled 30 min before, just before, immediately after, and 30 min after the beginning of the test. OPO reactivity to the test between smokers and non-smokers was measured in addition to uric acid concentration, flow rate, IgA, thiocyanate (SCN-) concentration, amylase activity as a salivary stress marker, and ultra-weak chemiluminescence (UCL) level, which is indicative of salivary antioxidative and antibacterial abilities. Moreover, we studied the effect of smoking on the response of salivary peroxidase (SPO) and myeloperoxidase (MPO) activity to mental stress, respectively. The results showed that the IgA concentration, amylase activity, SCN(- concentration, and UCL level are higher in the non-smoking group than smoking group and the IgA concentration and UCL level increased in the non-smokers significantly just after the Kraepelin test. The levels of SCN-) were higher in smokers than in non-smokers and OPO activity was greater in the non-smoking group in all sessions. Furthermore, only the non-smokers had significantly increased MPO activity just after the test. MPO may play a crucial role in the response to acute psychological stress besides inflammation, and CS suppresses this response significantly.
唾液是人体最先接触到诸如香烟烟雾（CS）等外源性物质或气体的体液。本研究旨在探讨吸烟是否会影响口腔过氧化物酶（OPO）对精神压力的反应性。研究对象为39名不吸烟者和10名吸烟者。在实验中，采用克雷佩林心理诊断测试作为心理应激源，并在测试开始前30分钟、即将开始前、刚结束后以及结束后30分钟采集唾液。除了测量尿酸浓度、流速、免疫球蛋白A（IgA）、硫氰酸盐（SCN-）浓度、作为唾液应激标志物的淀粉酶活性以及指示唾液抗氧化和抗菌能力的超微弱化学发光（UCL）水平外，还测量了吸烟者和不吸烟者对测试的OPO反应性。此外，我们分别研究了吸烟对唾液过氧化物酶（SPO）和髓过氧化物酶（MPO）活性对精神压力反应的影响。结果显示，非吸烟组的IgA浓度、淀粉酶活性、SCN（-）浓度和UCL水平高于吸烟组，且在克雷佩林测试刚结束后，非吸烟者的IgA浓度和UCL水平显著升高。吸烟者的SCN（-）水平高于不吸烟者，并且在所有测试阶段，非吸烟组的OPO活性更高。此外，只有非吸烟者在测试刚结束后MPO活性显著增加。MPO可能除了在炎症反应中之外，在对急性心理压力的反应中也起着关键作用，并且香烟烟雾会显著抑制这种反应。
