Greabu Maria, Battino Maurizio, Totan Alexandra, Mohora Maria, Mitrea Niculina, Totan Cosmin, Spinu Tudor, Didilescu Andreea
Department of Biochemistry, Faculty of Dental Medicine, Carol Davila University of Medicine and Pharmacy, 8 Boulevard Eroilor Sanitari sect. 5, Bucharest, Romania.
Pharmacol Rep. 2007 Sep-Oct;59(5):613-8.
The effect of smoking is in our days a serious global public health problem of major concern. Incidence of oral squamous cell carcinoma (SCC) in cigarette smokers is four to seven times higher than in nonsmokers. There is a constant and direct attack of various cigarette smoke constituents on the oral epithelial cells, which gradually accumulate and cause malignant transformation. Saliva is the first biological fluid that encounters inhaled cigarette smoke (CS). We have studied the influence of CS on salivary antioxidant capacity, uric acid, amylase and LDH (lactate dehydrogenase). In our study both, gas and particulate phase of CS were tested separately, and possible antioxidant effect of pyridoxine on salivary components was examined. Our results indicate that exposure to both, gas and particulate phase of CS caused a statistically significant decrease in salivary uric acid, LDH and amylase activity. We have also studied the effect of vitamin C (10 mg/dl) and vitamin B6 (1 mM) during incubation of saliva in the presence of CS. The addition of vitamin C had a significant (p < 0.05) protective effect on salivary uric acid level (0.25 +/- 0.12 for saliva incubated with gas phase of CS vs. 0.65 +/- 0.12 for saliva incubated with gas phase of CS in the presence of vitamin C). Vitamin C was not able to maintain/restore the original uric acid level. In the presence of the gas phase, pyridoxine had no protective effect, neither on salivary uric acid level nor on the FRAP activity of saliva. The purpose of our study was to discover a connection between the level of antioxidants in saliva in the presence of the two components of CS. Our results show that salivary antioxidant system is significantly and distinctly affected by both gas and particulate phase of CS and suggest that an adequate intake of antioxidants may help smokers to avoid CS-induced oxidative damage and to prevent degenerative diseases.
如今，吸烟的影响是一个严重的全球公共卫生问题，备受关注。吸烟者患口腔鳞状细胞癌（SCC）的几率比不吸烟者高4至7倍。香烟烟雾的各种成分持续直接侵袭口腔上皮细胞，这些细胞逐渐积累并引发恶性转化。唾液是最先接触吸入香烟烟雾（CS）的生物流体。我们研究了CS对唾液抗氧化能力、尿酸、淀粉酶和乳酸脱氢酶（LDH）的影响。在我们的研究中，分别测试了CS的气相和颗粒相，并研究了吡哆醇对唾液成分可能的抗氧化作用。我们的结果表明，接触CS的气相和颗粒相均导致唾液尿酸、LDH和淀粉酶活性在统计学上显著降低。我们还研究了在CS存在的情况下，维生素C（10mg/dl）和维生素B6（1mM）在唾液孵育过程中的作用。添加维生素C对唾液尿酸水平有显著（p<0.05）的保护作用（与气相CS孵育的唾液尿酸水平为0.25±0.12，而在维生素C存在下与气相CS孵育的唾液尿酸水平为0.65±0.12）。维生素C无法维持/恢复原始尿酸水平。在气相存在的情况下，吡哆醇对唾液尿酸水平和唾液的铁还原抗氧化能力（FRAP）活性均无保护作用。我们研究的目的是发现CS的两种成分存在时唾液中抗氧化剂水平之间的联系。我们的结果表明，CS的气相和颗粒相均显著且明显地影响唾液抗氧化系统，并表明充足摄入抗氧化剂可能有助于吸烟者避免CS诱导的氧化损伤并预防退行性疾病。
